Three different models: the unstructured mechanistic black-box model, the input-output neural network-based model and the externally recurrent neural network model were used to describe the pyruvate production process from glucose and acetate using the genetically modified Escherichia coli YYC202 ldhA::Kan strain. The experimental data were used from the recently described batch and fed-batch e...

The production of value-added chemicals from the bioconversion of lignocellulose biomass has been considered a promising venture. In this study, microwave, alkali-pretreated empty fruit bunch (EFB) was used as the substrate, utilizing pelletized filamentous Rhizopus oryzae NRRL 395 and cellulolytic enzymes for lactic acid production in a fed-batch simultaneous saccharification and fermentation ...

A dynamic flux balance model based on a genome-scale metabolic network reconstruction is developed for in silico analysis of Saccharomyces cerevisiae metabolism and ethanol production in fed-batch culture. Metabolic engineering strategies previously identified for their enhanced steady-state biomass and/or ethanol yields are evaluated for fed-batch performance in glucose and glucose/xylose medi...

Cocultivation of cellulolytic and saccharolytic microbial populations is a promising strategy to improve bioethanol production from the fermentation of recalcitrant cellulosic materials. Earlier studies have demonstrated the effectiveness of cocultivation in enhancing ethanolic fermentation of cellulose in batch fermentation. To further enhance process efficiency, a semicontinuous cyclic fed-ba...

Catechol-O-methyltransferase (COMT, EC 2.1.1.6) plays a crucial role in dopamine metabolism which has intimately linked this enzyme to some neurodegenerative diseases, such as Parkinson's disease. In recent years, in the attempt of developing new therapeutic strategies for Parkinson's disease, there has been a growing interest in the search for effective COMT inhibitors. In order to do so, larg...

BACKGROUND Industrial processes for recombinant protein production challenge production hosts, such as the yeast Pichia pastoris, on multiple levels. During a common P. pastoris fed-batch process, cells experience strong adaptations to different metabolic states or suffer from environmental stresses due to high cell density cultivation. Additionally, recombinant protein production and nutrient ...

Dynamics of fed-batch biological stirred tank reactors are commonly described by a set of ordinary differential equations arising from mass balances of the biological species (microorganisms, enzymes, substrates, etc.) involved in the process. A basic difficulty for the application of modern control science to these processes lies in the fact that the models usually include kinetic parameters w...

Dynamics are inherent characteristics of batch processes, and they may exist not only within a particular batch, but also from batch to batch. To model and monitor such two-dimensional (2D) batch dynamics, two-dimensional dynamic principal component analysis (2D-DPCA) has been developed. However, the original 2D-DPCA calculates the monitoring control limits based on the multivariate Gaussian di...

An assessment of a number of different process flowsheets for bioethanol production was performed using dynamic model-based simulations. The evaluation employed diverse operational scenarios such as, fed-batch, continuous and continuous with recycle configurations. Each configuration was evaluated against the following benchmark criteria, yield (kg ethanol/kg dry-biomass), final product concent...

Design and analysis of optimal control strategies for three types of inhibitory fed-batch bioprocesses have been discussed. These are simple saccharification (SS) of starch to glucose, simple fermentation (SF) of derived glucose to lactic acid (LA) and simultaneous saccharification and fermentation (SSF) of starch to LA. Various optimal feeding strategies have been investigated for the SSF proc...