PURPOSE To characterize retinal morphology and visual system function in the zebrafish mutant fade out (fad) and to establish the mutant as a lower vertebrate model for Hermansky-Pudlak syndrome (HPS). METHODS Retinal morphology of fad larvae was examined between 3 and 9 days postfertilization (dpf) by standard histology, transmission electron microscopy, and immunohistochemistry examination....

Aldehyde oxidase (EC 1.2.3.1) has been purified by a modification of a previously reported procedure and the FAD prosthetic group has been removed by treatment with calcium chloride and calcium acetate. The deflavo enzyme so obtained is devoid of Wmethylnicotinamide oxygen reductase activity but can be reconstituted by a short incubation with FAD. The different activities of native and deflavo ...

The oxidation-reduction midpoint potentials, Em, of the FAD and active site disulfide couples of Escherichia coli thioredoxin reductase have been determined from pH 5.5 to 8.5. The FAD and disulfide couples have similar Em values and thus a linked equilibrium of four microscopic enzyme oxidation-reduction states exists. The binding of phenylmercuric acetate to one enzyme form could be monitored...

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With unwarranted enthusiasm, the popular press has embraced hypoglycemia as an explanation for a host of physical ailments. Although this has permitted purveyors of numerous "treatments" to do a brisk business, very little helpful and accurate information has been communicated about hypoglycemia. Lynn Bennion, a Stanford-based clinician, has written a book that provides the general public acces...

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