The methods of extraction, separation and analysis of alkaloids and indole glucosinolates (GLs) ofIsatis tinctoria were reviewed. Different analytical approaches such as High-pressure Liquid Chromatography (HPLC), Liquid Chromatography with Electrospray Ionization Mass Spectrometry (LC/ESI/MS), Electrospray Ionization Time-Of-Flight Mass Spectrometry (ESI-TOF-MS), and Nuclear Magnetic Resonance...

Hydrochloric acid extraction of feces in the presence of ether yields an extract suitable for spectrophotometric estimation of total porphyrin and for further separation by "high-performance" liquid chromatography (HPLC) or thin-layer chromatography. A total porphyrin reference interval of less than 200 nmol/g dry weight of feces was established from data on 106 normal subjects on an unrestrict...

A model system for selective solubilization and fast separation of proteins from the rat liver membrane fraction and purified rat liver plasma membranes for their further proteomic analysis is presented. For selective solubilization, high-pH solutions and a concentrated urea solution, combined with different detergents, are used. After extraction, proteins are separated by anion-exchange chroma...

This work presents the investigation of various capillary electrophoretic techniques for the Separation of low-molecular-weight phenolic and neutral lignin degradation products. A successful CE mode was micellar electrlokinetic chromatography (MEKC) with carrier electrolytes containing micelleformi:ng reagents, such as sodium dodecylsulfate. Important phenolic and neutr;al lignin degradation co...

Methods for the separation of adrenaline, noradrenaline, hydroxytyramine, and dihydroxyphenylalanine (dopa) by employing starch columns (2, 3), paper chromatography (4-7), or countercurrent extraction (8) have limited usage either because of the length of time required for the fractionation or because of the small amounts of material that can be separated. A procedure is described below in whic...

− The molecular imprinting technique is a reliable technique for the preparation of materials of predetermined selectivity. It is highly significant in research of the structure of enzyme, mechanism of receptor-antibody and analytical chemistry. In analytical separation science, molecularly imprinted polymers have been applied in several analytical techniques, such as liquid chromatography, cap...

A quantitative HPLC – fluorescence method for the simultaneous determination of sulfamerazine (SMR) and sulfamethoxazole (SMX) in honey has been developed. Sample pretreatment included acidic hydrolysis, followed by solid phase extraction (SPE) procedure. The liquid chromatography (LC) separation was carried out on RP C 18 column and sulfonamides were detected by fluorescence detector after pre...

Grape anthocyanins not only play an important role in the colour quality of red wines but they also have many beneficial effects on human health, e.g., reduction of coronary heart disease incidence, or anticarcinogenic and antioxidant properties. Therefore, a rapid and efficient extraction technique prior to chromatographic analysis is of primary interest. Pressurised Fluid Extraction (PFE) pre...

A quantitative, specific, and sensitive method for the determination of N-acylhomoserine lactones (HSLs - a group of bacterial semiochemicals) in the form of their hydrolysis products (N-acylhomoserines, HSs) is presented. Real samples were analyzed by capillary zone electrophoresis-mass spectrometry (CZE-MS) after alkaline lactonolysis and extraction by mixed-mode anion-exchange solid-phase ex...

Waste oil industry has a great potential use, as 77% oil yield are an untapped waste that still has a high economic value as a base for the oleochemical industry, one of which is β-carotene. The study was conducted to optimize the extraction of carotenoids from Crude Palm Oil (CPO) and Palm Oil Mill Effluent waste (POME) conducted by column chromatography and extraction method with a single enz...