Stabilizing extracellular enzymes may maintain enzymatic activity while protecting enzymes from proteolysis and denaturation. A study determined whether a fast pyrolysis hardwood biochar (CQuest™) would reduce evaporative losses, subsequently stabilizing soil extracellular enzymes and prohibiting potential enzymatic activity loss following a denaturing stress (microwaving). Soil was incubated i...

Lecanicillium muscarium CCFEE 5003, isolated in Continental Antarctica, is a powerful producer of extracellular cold-tolerant enzymes. Chitin-hydrolyzing enzymes seems to be the principal extracellular catalytic activities of this psychrotolerant fungus. The production of chitinolytic activities is induced by chitin and other polysaccharides and is submitted to catabolite repression. The chitin...

High salt-tolerant extracellular protease is an enzyme that plays a crucial role in industries and requires high salt conditions. Some enzymes are not resistant to conditions because they may denature under concentrations. Halophilic microbes produce stable at salts. This research has succeeded isolating several halophilic capable of producing proteases from Bledug Kuwu, Grobogan, Central Java,...

Lysosomes are organelles that support diverse cellular functions such as terminal degradation of macromolecules and nutrient recycling. Additionally, lysosomes can fuse with the plasma membrane, a phenomenon referred to lysosomal exocytosis, release their contents, including hydrolytic enzymes cargo proteins. Recently, neuronal activity has been shown induce exocytosis in dendrites axons. Secre...

Extracellular soil enzymes play a key role in organic matter decomposition and nutrient cycling. However, it is not fully understood how these respond to different land use. Long-term research studies were used evaluate diversified management practices affect extracellular driving C cycling [phenol oxidases (PO), peroxidases (PP), α-glucosidase (AG), β-glucosidase (BG), cellobiohydrolase (CB), ...

BACKGROUND Neutropenia, as a predisposing factor for invasive candidiasis, is defined as a reduction in neutrophil count to less than 1500/mm3. It is a common condition in patients with hematological malignancy and cytostatic chemotherapy. Extensive chemotherapy and prophylaxis with antifungals have increased the resistance of Candida isolates to antifungal drugs. Although, Candida albicans is ...

background one group of significant enzymes produced by bacillus genus are alkaline proteases with several important applications in the daily life as well as common industries such as food, detergents, leather, alcohol and beer production and medical and sanitary industries, beside wastewater treatment, , biotransformation, hydrolyzed proteins and oil manufacturing. objectives in this paper, k...

Extracellular enzymes are ubiquitous in soil environments. Produced by microorganisms and plant roots, these enzymes serve a dual function of degrading complex organic material into simpler forms and acquiring resources for the enzyme producer (Burns 1982; Sinsabaugh 1994). Without extracellular enzymes, microbes and plants would be unable to obtain resources from complex compounds, and cycles ...

This study investigates the mechanisms as well as strategies for purification and characterization of potential enzymes involved in pathogenesis of entomopathogenic fungi. The test strain of Verticillium lecanii that was screened, during the present investigation, proved to be an efficient producer of protein and polysaccharide degrading enzymes (amylase, protease, and lipase), hence indicating...

The gelatinase, urease, lipase, phospholipase and DNase activities of 11 chromoblastomycosis agents constituted by strains of Fonsecaea pedrosoi, F. compacta, Phialophora verrucosa, Cladosporium carrionii, Cladophialophora bantiana and Exophiala jeanselmei were analyzed and compared. All strains presented urease, gelatinase and lipase activity. Phospholipase activity was detected only on five o...