Bacteria that simultaneously produce multiple extended-spectrum beta-lactamases are frequently isolated. We report an Enterobacter cloacae isolate, ES24, producing four different beta-lactamases (AmpC type beta-lactamase, TEM-1, SHV-7, and a novel extended-spectrum beta-lactamase, SHV-30). Direct sequencing of bla(SHV) gene products gave a "double peak" at position 703, suggesting the presence ...

The NitroSpeed-Carba NP test was used to rapidly detect and discriminate between the different types of carbapenemases (classes A, B, D) within 30 minutes among a collection 202 Pseudomonas sp. strains (mostly aeruginosa). A total 99 carbapenemase–(including enzymes exhibiting weak carbapenemase activity such as several Guyana Extended-Spectrum (GES)-ß-lactamases) 103 non-carbapenemase producer...

The first betalactamases were identified in a species of E.coli in 1940 (1). However, the ability of bacteria to produce enzymes that destroy the b-lactam ring was noted even before penicillin was developed. In fact, many of the gram-negative bacteria possess chromosomally mediated blactamases, which help the bacteria find a niche when faced with competition from other bacteria that naturally p...

The occurrence of multidrug-resistant Serratia marcescens strains producing metallo-β-lactamases or extended-spectrum β-lactamases represents a serious public health threat. Here, we report the draft genome sequence of a multidrug-resistant carbapenemase-producing Serratia marcescens isolate recovered from the bronchoalveolar lavage specimen of a patient suffering from chronic obstructive pulmo...

A clinical Providencia stuartii isolate SM662 was recovered from a patient hospitalized in the intensive care unit at the Military hospital, Tunisia. This isolate was resistant to penicillins, cephalosporins, aminoglycosides and fluoroquinolones. A marked in vitro synergy between ceftazidime or cefotaxime and amoxicillin-clavulanic acid on Mueller-Hinton agar plates suggested the presence of an...

Background Extended spectrum beta-lactamases (ESBLs) are enzymes produced by some gram negative bacilli that mediate resistance to extended-spectrum cephalosporins and aztreonam. ESBLs are commonly recognized in a variety of Enterobacteriaceae and Pseudomonas aeruginosa isolates. This study focused on the prevalence of ESBL producing strains from diabetes patients and their antimicrobial correl...