Emergence of extended spectrum β lactamases (ESBLs) producing strains of gram negative bacteria, as one of the leading cause of septicemia often complicates the clinical and therapeutic outcome. The present study was undertaken to investigate the prevalence of ESBLs in bacteria isolated from neonatal septicemia cases along with their antimicrobial sensitivity pattern. Blood samples were collect...

The Oxoid combination disk method for detecting extended-spectrum beta-lactamases (ESBLs) depends on comparing the inhibition zones of cefpodoxime (10-microg) and cefpodoxime-plus-clavulanate (10- plus 1-microg) disks. The presence of clavulanate enlarged the zones for all of 180 ESBL-producing klebsiellae by >/=5 mm, whereas zones for cefpodoxime-susceptible isolates and cefpodoxime-resistant ...

conclusions: this study highlighted the need to establish antimicrobial resistance surveillance networks for p. aeruginosa to determine the appropriate empirical treatment regimens. the high prevalence of multidrug resistance and production of esbls in p. aeruginosa isolates confirms the necessity of protocols considering these issues in the hospitals. results: the resistance against broad-spect...

resistance to oxyimino cephalosporins antibiotics in enterobacteriaceae is primarily done by the extended spectrum β-lactamases (esbls). clear identification of risk factors for esbl-producing infections is necessary. therefore, efficient strategies can be developed to decrease outbreak of these infections. the aim of this study was to determine the antibacterial susceptibility and esbls patter...

results of the 100 klebsiella isolates, 41 (41%) demonstrated resistance or reduced susceptibility to ceftazidime and/or aztreonam and 35% (n = 35) were esbl-producers. twenty-eight (8o%) of the esbl-producing isolates carried the blactx-m type genes. based on pcr assays and sequencing of blactx-m genes, ctx-m-1, ctx-m-2 and ctx-m-9 were identified in 21 (60%), 15 (42%) and nine (34%) of these ...

Respiratory isolates of Klebsiella pneumoniae in Korea during 2002-2003 were studied to determine the prevalence and types of extended-spectrum beta-lactamases (ESBLs) and plasmid-mediated AmpC beta-lactamases (PABLs). ESBL-production was tested by double-disk synergy, and genotypes of beta-lactamases were determined by PCR and sequencing. ESBLs were detected in 28.4% of 373 isolates, and the m...

Many clinical laboratories have problems detecting extended-spectrum beta-lactamases (ESBLs) and plasmid-mediated AmpC beta-lactamases. Confusion exists about the importance of these resistance mechanisms, optimal test methods, and appropriate reporting conventions. Failure to detect these enzymes has contributed to their uncontrolled spread and sometimes to therapeutic failures. Although Natio...

The production of β-lactamase is the principal mechanism by which gram-negative bacteria resist the action of β-lactam antibiotics. In recent decades, there has been an alarming explosion in the diversity, global dissemination, host range, and spectrum of activity of β-lactamases. This has been most clearly reflected by the marked increase in infections caused by bacteria that express extended-...

Microbial drug resistance is a growing problem of global magnitude. In gram-negative pathogens, the most important resistance problems are encountered in Enterobacteriaceae, Pseudomonas aeruginosa and Acinetobacter, with increasing trends observed for all major anti-gram-negative agents (beta-lactams, fluoroquinolones and aminoglycosides). A matter of major concern is the emergence of new beta-...

Objective: The aim of the study is to determine Extended-Spectrum β-Lactamases (ESBLs) by three different phenotypic methods Escherichia coli (E. coli) strains that isolated from various clinical samples. 
 Methods: A total 93 E. samples were hospitalized patients. Antibiotic susceptibility tests done automated system Phoenix 100 (Becton Dickinson, Sparks, MD, USA). ESBL production was tes...