The natural tragacanth gum, the sap of Astragalus gummifer, has been successfully used as a gelling agent in tissue culture media for in vitro shoot development and proliferation of miniature rose and carnation. Shoot development of nodal segments excised from greenhouse grown plants, was investigated on MS medium solidified with either 2.5, 3.0, 3.5 and 4.0% tragacanth or 0.8% agar as control....

Abstract Background and Objectives Proliferation and differentiation of mesenchymal stem cells (MSCs) in culture media requires the presence of nutrient supplements such as bovine fetal serum (FBS), which increases the risk of prion/xenotic infections. Platelet lysates as a rich source of growth factors and cytokines can replace FBS in cell culture medium. This study aimed to compare the effe...

the growing demand for animal products has made importance of the utilization of biotechnological techniques in improvement of forage crops such as sainfoin. in this research, the effects of different levels of auxin (naa and iba) and cytokinin (bap, zeatin and tdz) on in vitro growth response of shoot apex explants of shahrkord and khansar cultivars of sainfoin were investigated. for this, sho...

Leaves from in vitro proliferating shoots of cvs. Chandler and Hartley of Persian walnut were cultured on MS and NN media supplemented with BA and NAA. Three light conditions including darkness, low light intensity and modified light intensity were used. Most of the calli were friable and non-embryonic in all three conditions. Rate of callogenesis depended on cultivar, culture medium, light con...

Fresh or dry naked seed-explants of different maturity were cultured in vitro and their responses were investigated on a medium containing 2mg/l 2,4-dichlorophenoxyacetic acid (2,4-D) as growth regulator. It was found that the explants at lower maturity had lower rates of callus induction and lower production of callus masses than those with the more matured ones in the original cultures. Howev...

Apple is one of the most important temperate-zone fruit trees in the world. Micropropagation of apple scion and rootstocks is an important method of rapid production of healthy and disease-free plants. In this research, in vitro propagation of three apple roottocks including Azayesh-Esfahan, Morabbaee-Mashhad and M9 was investigated. Shoot proliferation carried out in two basal media (MS and WP...

Lisianthus (Eustoma grandiflorum L.) is a very popular, ornamental plant. Due to low germination of its seeds, usual techniques for its propagation are not efficient. The present study was aimed to establish callus from explants of different plant parts of Eustoma grandiflorum L. Raf Shinners in B5, LS and MS media with modifications.  The results showed that among different plant parts, leaf e...

The aim of this study was to develop methods for the in vitro propagation of Zantedeschia rehmannii. Zantedeschia rehmannii tuber fragments (1 cm2) containing eyes were soaked for 30 s in a solution containing 100 mg dm-3 L-ascorbic acid (AA) before transfer to culture vessels containing an MS medium supplemented with BAP (0 to 3 mg dm-3). Cultures were maintained in darkness. Soaking explants ...

pollution arising from plastic waste materials caused a great worry for researchers who are interested in protection of environment. this problem shifts their subject to bio-polymers for applications in packaging industries. however, their low water barrier properties are a critical problem. the effects of citric acid and methyl cellulose on water vapor and oxygen permeabilities and mechanical ...