Brassavola nodosa (L.) Lindl. is a tropical epiphytic orchid showing characteristics of interest for the ornamental nursery industry, as well considered an endangered species. Problems with traditional propagation methods limit development large-scale commercial production system. The use micropropagation has been investigated this species, and liquid in vitro systems showed potential temporary...

In vitro rooting response and indole-3-acetic acid (IAA) levels were examined in two genetically related dwarfing apple (Malus pumila Mill) rootstocks. M.26 and M.9 were cultured in vitro using Linsmaier-Skoog medium supplemented with benzyladenine (BA), indole-3-butyric acid (IBA), and 1,3,5-trihydroxybenzoic acid (PG). Rooting response was tested in Lepoivre medium supplemented with IBA and P...

A suitable protocol for micropropagation of Casuarina hybrid, Casuarina equisetifolia L. 9 Casuarina glauca Sieber ex Spreng (C. e. 9 C. g.), was developed. When seeds without seed coats were cultured on 4 germination media, the optimal seed germination percentage (91%) was obtained on 0.8% agar solidified water medium. Shoot multiplication was achieved by culturing 2-cm long epicotyls, excised...

ABSTRACT: Effective protocols for in vitro rooting woody fruit trees are still a challenge seedling production, especially when there is need to insert new cultivars or rootstocks. These essential accelerate studies plant breeding programs and distribution. This study evaluated the use of 6-Benzylaminopurine (IBA) Pyruscomunnis rootstocks, clones ‘OHxF87’ Pyrodwarf. Explant exposure times (0, 2...

Dittany of Crete (Origanum dictamnus L.) is a threatened medicinal-aromatic plant Lamiaceae family which local endemic to the Island Crete, Greece. Its high culinary use and increasing demand in pharma, perfumery, cosmetic food industry along with its overexploitation from natural habitat has this species necessitated large-scale production for industrial exploitations using advanced technologi...

In vitro regeneration of plants from root cultures of Swertia chirata Buch.-Ham. ex Wall was obtained. Root explants from axenic shoot cultures were used for shoot induction. Optimal shoot regeneration without any callus intervention was observed on 1/2 MS (Murashige and Skoog’s medium, 1962) + 4.44 μM 6, benzylaminopurine (BAP) +1.07 μM α-naphthalene acetic acid (NAA).Regenerated shoots were f...