We have studied the M cell origin and differentiation pathway in rabbit gut-associated lymphoid tissues. Micro-dissected domes and epithelium isolated by ethylene diamine tetra acetic acid detachment allowed us to view the whole epithelial surface from the bottom of crypts to the top of domes. We used monoclonal antibodies specific to the apex of either M cells or dome enterocytes, lectins, and...

INTRODUCTION Particle-emitting, bone-seeking radiopharmaceuticals have attracted the attention of the nuclear medicine community over the last three decades for the treatment of the pain of osteoblastic metastases. The objectives of this research were to produce quality-controlled (159)Gd-EDTMP in order to provide a new therapeutic radiopharmaceutical for use in clinical applications. METHODS...

OBJECTIVE To determine the reference ranges of lymphocyte subsets in serologically HIV-seronegative healthy male adults in Oman. METHODS A cohort, of 118 healthy male blood donors ranging in age from 18-51 years, was included in the study. The average age was 25 years. Blood samples collected into tubes containing ethylene-diamine-tetra acetic acid were investigated for lymphocyte subsets usi...

INTRODUCTION Today, with the tremendous potential of genomics and other recent advances in science, the role of science to improve reliable DNA extraction methods is more relevant than ever before. The ideal process for genomic DNA extraction demands high quantities of pure, integral and intact genomic DNA (gDNA) from the sample with minimal co-extraction of inhibitors of downstream processes. ...

Enterocins are small, heat stable and antimicrobial agents are directed against a broader spectrum of bacteria. Isolation, partial characterization and antibacterial activity of partially purified enterocin from Enterococcus faecalis form the aim of this study. The protein content of crude enterocin of the enterocin from highest producing strain was 16.6 mg/ml and its activity was 170 AU/ml. Th...

Absorption: With the original technique (1) the horse serum decomplemented by heating to 56 0 C. and then absorbed against cells and pooled serum of the species to be treated; tion was done first to avoid hemolysis of the added red cedure had two flaws. First, the use of serum (rather than p.,,"~ ... aJ ICtJ,:'lgror absorption precluded the removal of antifibrinogen and bocyte antibodies. Secon...

Two surfactant lipid preparations (SLPs) were investigated to determine their mechanism of antimicrobial action. 8N8, a water-in-oil emulsion, and W60C, a liposome, both have bactericidal activity against Gram-positive bacteria and non-enteric Gram-negative bacteria. Additionally, W60C is bactericidal for enteric Gram-negative bacilli when suspended in deionized water. Zeta potential measuremen...

Melia dubia Cav. of family Meliaceae is a fast growing, high value tree species native to India. Isolating DNA from matured dried leaves of M. dubia was difficult due to accumulation of secondary metabolites, majorly polyphenolics, which resulted in dark brown to black colour of the pellet. In this study, a modified STE-(Sucrose, Tris-HCl and Ethylene Diamine Tetra Acetic Acid) CTAB (hexadecylt...

Antibodies were prepared against carboxydismutase and latent Ca2® dependent ATPase (coupling factor) purified from Nicotiana tabacum. The antibodies against carboxydismutase inhi­ bit the enzymatic activity of the purified protein, while those against coupling factor inhibit the Ca2© dependent ATPase activity of the protein as well as the photophosphorylation of the chloro­ plasts. The antisera...