Extracellular carboxylic ester hydrolases are produced by many bacterial pathogens and have been shown recently to be important for virulence of some pathogens. However, these hydrolases are poorly characterized in enzymatic activity. This study prepared and characterized the secreted ester hydrolase of Streptococcus equi ssp. equi (designated SeE for S. equi esterase). SeE hydrolyzes ethyl ace...

The antitumor properties of (E)-2-(fluoromethyl)dehydroornithine methyl ester (A-MFMO-ME) and of (E)-2-(fluoromethyl)dehydroornithine ethyl ester (A-MFMO-EE), the prodrugs of A-MFMO, an irreversible inhibitor of mammalian L-ornithine decarboxylase (ODC) 14 times more potent than a-difluoromethylornithine (DFMO) and equipotent to (2R,5R)-6-heptyne-2,5-diamine (MAP) in vitro, have been inves tiga...

A mixture of steamed soybean and boiled rice with seeded Aspergillus oryzae was naturally fermented without addition of yeasts or Lactobacilli, and kept matured for 12 months at room temperature. Chemical analysis of this rice-koji miso sample for lipid changes during maturation showed that triacylglycerol was gradually decomposed into free fatty acid, with distinct formation of fatty acid ethy...

The serine protease inhibitors diethyl p-nitrophenyl phosphate and phenylmethylsulfonyl fluoride (chemical modifiers of serine residue) and N-acetyl-l-tryptophan ethyl ester (competitive inhibitor of chymotryptic protease) inhibited 1-O-alkyl-2-acetyl-sn-glycero-3-phosphocholine (AGEPC; platelet-activating factor)-induced platelet aggregation and secretion. The inhibition was dependent on the p...

The common use of dicumarinyl derivatives for the treatment of thromboembolic diseases justifies further clinical evaluation of dicumarol and 4,4'-dihydroxydicumarinyl ethyl acetate [Pelentan, Tromexan, 3,3'-carboxymethylenebis (4-hydroxycoumarin) ethyl ester.] The need for a more thorough understanding of the pharmacodynamic action of dicumarinyl anticoagulants has prompted a study of their gr...

Pre-steady state kinetic parameters pertaining to chymotrppsin-catalyzed hydrolysis of specific substrate esters can be obtained from measurements of the displacement of the competitive inhibitor proflavin from enzyme by substrate. This method, which was previously used in experiments with specific substrate amides, has now been applied to the investigation of N-acetyl-L-phenylalanine methyl es...

In the title compound, C(32)H(40)N(4)O(4), the pyrrole rings and ester groups adopt a 1,3-alternate conformation in which the alternating pyrrole and ester units are in opposite directions. The structure displays N-H⋯O hydrogen bonding and exhibits disorder [site occupancies of 0.81(2) and 0.71(2)] in the peripheral ethyl groups.