Trichoderma reesei can be considered as a candidate for consolidated bioprocessing (CBP) microorganism. However, its ethanol yield needs to be improved significantly. Here the ethanol production of T. reesei CICC 40360 was improved by genome shuffling while simultaneously enhancing the ethanol resistance. The initial mutant population was generated by nitrosoguanidine treatment of the spores, a...

BACKGROUND There is currently a steady increase in the use of wood-based fuels for heat and power production in Sweden. A major proportion of these fuels could serve as feedstock for ethanol production. In this study various options for the utilization of the solid residue formed during ethanol production from spruce, such as the production of pellets, electricity and heat for district heating,...

BACKGROUND Efficient xylose fermentation by yeast would improve the economical and sustainable nature of biofuels production from lignocellulosic biomass. However, the efficiency of xylose fermentation by the yeast Saccharomyces cerevisiae is suboptimal, especially in conversion yield, despite decades of research. Here, we present an improved performance of S. cerevisiae in xylose fermentation ...

A nascent cellulosic ethanol industry is struggling to become cost-competitive against corn ethanol and gasoline. Millions of dollars are spent on nitrogen supplements to make up for the low nitrogen content of the cellulosic feedstock. Here we show for the first time to our knowledge that the ethanol-producing bacterium, Zymomonas mobilis, can use N2 gas in lieu of traditional nitrogen supplem...

A previous study demonstrated that paper sludges with high ash contents can be converted to ethanol by simultaneous saccharification and fermentation (SSF) or simultaneous saccharification and cofermentation (SSCF). High ash content in the sludge, however, limited solid loading in the bioreactor, causing low product concentration. To overcome this problem, sludges were de-ashed before SSF and S...

Receipt 10 January 2003, accepted 28 April 2003. Abstract The efficient methods for extracting phenolics from grape seeds have been of great interest to many investigators, due to the special health promoting and disease preventing effects of polyphenols. In this study, extractions were performed by using aqueous ethanol. The effects of single factors such as the concentration of ethanol, the r...

Current technology using corn stover (CS) as feedstock, Ammonia Fiber Expansion (AFEX) as the pretreatment technology, and Saccharomyces cerevisiae 424A(LNH-ST) as the ethanologenic strain in Separate Hydrolysis and Fermentation was able to achieve 191.5 g EtOH/kg untreated CS, at an ethanol concentration of 40.0 g/L (5.1 vol/vol%) without washing of pretreated biomass, detoxification, or nutri...

This study presents bioconversion of water hyacinth (Eichhornia crassipes) to bioethanol using two-sequential steps of acid hydrolysis (10% sulfuric acid) and yeast (Candida intermedia) fermentation. Maximum ethanol yield co-efficient was comparable to those obtained through enzymatic saccharification and fermentation of acid hydrolysate using fully equipped fermentor. A maximum ethanol yield (...

Bioethanol is one of the most important alternative renewable energy sources that substitute the fossil fuels. Sugarcane bagasse has a content of cellulose and hemicelluloses, which make it suitable as fermentation substrate when hydrolyzed. The objective of work is ethanol production from sugarcane bagasse (SCB) by the fermentation process. Eight laboratory experiments were conducted to produc...

This paper presents a thermodynamic analysis of a high-yield biochemical process for biofuel production from lignocelluosic biomass based on a previously proposed process. Unlike the standard biochemical process, which ferments sugar intermediates to ethanol, the process under consideration converts sugars to acetic acid which is esterified and hydrogenated to produce ethanol. This process has ...