Chylomicron triglyceride and lecithin obtained after feeding mixtures of three or four free fatty acids to rats were hydrolyzed using pancreatic lipase and phospholipase A respectively. The distribution of fatty acid mass and radioactivity in the substrate materials and in the cleavage products was determined by gas-liquid chromatography. The incorporation of exogenous (labeled) fatty acids int...

We examined brain phospholipid metabolism in mice in which the cytosolic phospholipase A(2) (cPLA(2,) Type IV, 85 kDa) was knocked out (cPLA(2)(-/-) mice). Compared with controls, these mice demonstrated altered brain concentrations of several phospholipids, reduced esterified linoleate, arachidonate, and docosahexaenoate in choline glycerophospholipid, and reduced esterified arachidonate in ph...

Despite their important role in tissues, fluids and foods, the analysis of non-esterified fatty acids (NEFA) as methyl esters (NEFAME) is performed using expensive, cumbersome and time-consuming procedures that needs of isolation, fractionation and derivatization steps. However, Yi et al. [1] proposed a promising in situ, single-step procedure to analyze esterified fatty acids (EFA) and NEFA fr...

The effects of Greyhound racing on plasma insulin, glucagon and non-esterified fatty acid have not been extensively studied. We sought to examine the effects of exercise in 19 greyhounds trialed over 400 meters on serum biochemistry, osmolality, nonessential fatty acids, and the major glucoregulatory hormones insulin and glucagon, before and after racing. Due to the non-traditional dietary patt...

The ability of a series of 18 carbon acetylenic fatty acids to fulfill the unsaturated fatty acid requirements of Escherichia coli and Saccharomyces cerevisiae was investigated. Despite their high melting points (greater than 40 degrees C), several isomers of the acetylenic fatty acids were as efficient or more efficient in supporting growth than the analogous fatty acid having a cis-double bon...

Carboxylic acid esters react with hydroxylamine in alkaline solution to form hydroxamic acids. These acids produce a red to violet reaction with ferric chloride which has been utilized by Feigl (1949) for the microchemical detection of carboxylic acid derivatives. Lipmann and Tuttle (1945) developed a method for the quantitative assay of acylphosphates by allowing hydroxylamine to react with th...

BACKGROUND Medium chain length (C6-C12) α,ω-dicarboxylic acids (DCAs) and corresponding esters are important building blocks for the polymer industry. For DCAs of 12 carbon atoms and longer, a sustainable process based on monooxygenase catalyzed ω-oxidation of fatty-acids has been realized. For medium-chain DCAs with a shorter chain length however, such a process has not been developed yet, sin...

The distribution of long-chain saturated fatty acids in triglycerides is different in infant formulas to that in human milk. In human milk, palmitic acid is predominantly esterified in the sn-2 position (beta-position) of the triglycerides, whereas in infant formulas, it is esterified mainly in the sn-1,3 positions (alpha,alpha'-positions). The specific distribution of the fatty acids in the tr...

1. The effect of infusion of noradrenaline (0.42 mumol min-1 kg-1) on the exchange of nonesterified fatty acids, glycerol and other metabolites across subcutaneous abdominal adipose tissue was investigated in five healthy subjects using an arteriovenous catheterization technique and measurement of adipose tissue blood flow using the 133Xe clearance technique. At the same time, the net rate of f...