A multiplex PCR (MPCR) for detection of vanA-and vanB-mediated resistance to vancomycin was optimized and adapted for use in the routine microbiology laboratory. Consecutive specimens (1196) submitted for vancomycin resistant Enterococci (VRE) surveillance were processed by clinical technologists on Bile Esculin Azide Agar containing 6 mg/L vancomycin (BEAA/Vanco6) plates and 466 showing black ...

Bacterial samples had been isolated from clinically detected diseased juvenile Pangasius, collected from Mymensingh, Bangladesh. Primarily, the isolates were found as Gram-negative, motile, oxidase-positive, fermentative, and O/129 resistant Aeromonas bacteria. The species was exposed as Aeromonas hydrophila from esculin hydrolysis test. Ten isolates of A. hydrophila were identified from eye le...

A recent paper published by Nieves-Morión and colleagues proposes that the intercellular transfer of small molecules in heterocyst-forming cyanobacteria (calcein, 5-carboxyfluorescein [5-CF], and esculin) is mediated by simple diffusion based on the Q10 values of the kinetics (1). In this letter, we show our analysis of their results to provide a thermodynamic basis of the kinetics. We obtained...

Vitis amurensis Ruprecht contains a large number of polyphenolic compounds which are biologically active components. For the most efficient and safe extraction supercritical carbon dioxide was used. In this work, for first time, comparative metabolomic study substances wild grapes collected from five different places Primorsky Khabarovsk territories is carried out. To identify target analytes i...

The objective was to describe and compare antimicrobial resistance patters of esculin-hydrolyzing streptococci streptococcal-like organisms (Streptococcus uberis, Enterococcus faecium, faecalis, Lactococcus garvieae, lactis) from routine diagnostic samples the udder health laboratory Bavarian Animal Health Services between 2015 2019. All Services, that were tested with a standard microbroth dil...

Thermotoga neapolitana beta-glucosidase (BglA) was subjected to site-directed mutagenesis in an effort to increase its ability to synthesize arbutin derivatives by transglycosylation. The transglycosylation reaction of the wild-type enzyme displays major beta(1,6) and minor beta(1,3) or beta(1,4) regioselectivity. The three mutants, N291T, F412S, and N291T/F412S, increased the ratio of transgly...

Campylobacter (Campy; BD Diagnostics, Sparks, MD), Spectra VRE (Remel, Lenexa, KS), and bile-esculin-azide-vancomycin (BEAV; Remel) agars were compared for their ability to detect vancomycin-resistant enterococci (VRE) in 750 stool specimens. The media were compared at 24 h and 48 h of incubation at 35°C and 42°C. When incubated for 24 h at 35°C, Campy was the most sensitive (97.8%) and specifi...

A test is described that could replace bacitracin susceptibility for presumptive identification of group A streptococci as well as 6.5% NaCl agar tolerance for presumptive identification of enterococcal streptococci. The L-pyrrolidonyl-beta-naphthylamide test, based on hydrolysis of pyrrolidonyl-beta-naphthylamide, was used in conjunction with the CAMP and bile-esculin tests to presumptively id...

A scheme has been developed for the identification of thermophilic actinomycetes associated with hypersensitivity pneumonitis. Eighty strains, 10 Micropolyspora faeni, 6 Saccharomonospora viridis, 52 Thermoactinomyces candidus, 7 T. vulgaris, 4 T. sacchari, and 1 T. dichotomica, either isolated from patients' environment or received as authentic strains, were studied. In addition to the cultura...

A new strain, GH9, having ß-glucosidase activity was isolated from the soil of wild ginseng using Esculin-R2A agar. It shows the strongest activities to convert ginsenoside Rb1 to minor ginsenosides compound K. The transformation products were identified by thin layer chromatography (TLC) and high performance liquid chromatography (HPLC), and strain GH9 was found to transform major ginsenoside ...