background: we have previously reported that a 31 kda protein was screened from the excretory-secretory (es) proteins of tichinella spiralis muscle larvae (ml) by immunoproteomics using early infection sera, and the gene encoding a 31 kda protein from t. spiralis was cloned and expressed in an e. coli expression system. in this study, the recombinant 31 kda antigens were used for detection of a...

Transcription factor Lbx1 is known to play a role in the migration of muscle progenitor cells in limb buds and also in neuronal determination processes. In addition, involvement of Lbx1 in cardiac neural crest-related cardiogenesis was postulated. Here, we used mouse embryonic stem (ES) cells which have the capacity to develop into cells of all three primary germ layers. During in vitro differe...

Background: Transgenic mice are being considered as invaluable tool in biological sciences towards comprehension of the cause of the genetic diseases. Manipulated embryonic stem (ES) cells are used to produce loss-of-function mutant mice. Microinjection of manipulated ES cells into blastocoel cavity, and morula fusion are the two main techniques in producing transgenic mice. So...

The way to overcome current limitations in the generation of glucose-responsive insulin-producing cells is selective enrichment of the number of definitive endoderm (DE) progenitor cells. Sox17 is the marker of mesendoderm and definitive endoderm. The aim of the present research was to study the potential of Sox17 knock-in CGR8 mouse embryonic stem (ES) cells to differentiate into insulin produ...

Cardiac contractility was studied in a clinically relevant conscious swine model simulating human hemodynamics during endotoxemia. The slope of the end-systolic pressure-volume relationship [end-systolic elastance ( E ES)] was used as a load-independent contractility index. Chronic instrumentation in 10 pigs included two pairs of endocardial ultrasonic crystals for measuring internal major and ...

We have attempted to generate embryonic stem (ES) cell-derived hepatocytes expressing liver-specific functional properties by use of ES cell technology. It was found that ES cells are allowed to differentiate into hepatocytes possessing high metabolic activities when hepatocyte nuclear factor (HNF)-3β-transfected ES cells are cultured in α-MEM medium supplemented with 10% fetal bovine serum (FB...

Casella et al. [2, (2009)] proved that, under very general conditions, for normal linear models the Bayes factor for a wide class of prior distributions, including the intrinsic priors, is consistent when the number of parameters does not grow with the sample size n. The special attention paid to the intrinsic priors is due to the fact that they are nonsubjective priors, and thus accessible pri...

Wikis lassen sich für vielerlei Zwecke verwenden. In diesem Beitrag werden einige Aspekte der an einem Gymnasium durchgeführten Erprobung eines Wikis auf seine Eignung als Lernumgebung für den Informatikunterricht dargestellt. Der Schwerpunkt liegt dabei auf Fragen der Unterstützung rollenbasierten kollaborativen Lernens und der Darstellung der mit dem System gemachten Erfahrungen. Es zeigt sic...