conclusions the results revealed that the novel multi-epitope dna construct could be an effective candidate in tuberculosis vaccine development, and it is qualified to investigate its potential to induce cd4 and cd8 t- cell immune response in the experimental animal model. results mhc class i-and class ii-predicted epitopes showed a high potential to binding to human hlas alleles, with global b...

B-cell epitopes are special regions of antigens recognized by the binding sites of immunoglobulin molecules (Van Regenmortel, 1993). These epitopes play an important role in vaccine design, disease diagnosis, and allergy research (Saha and Raghava, 2006). Although many experimental techniques have been widely used to detect B-cell epitopes, the process is both time-consuming and laborintensive....

House dust mite (HDM) allergy is the leading cause of IgE-mediated hypersensitivity. Therefore identifying potential epitopes in the Dermatophagoide pteronyssinus 23 (Der p 23), a major house dust mite allergen will aid in the development of therapeutic vaccines and diagnostic kits for HDM allergy. Experimental methods of epitope discovery have been widely exploited for the mapping of potential...

We have investigated mechanisms involved in immunodominance of the CTL response of C57BL/6 (B6) mice against cells of BALB.B origin. This transplantation barrier consists of at least 40 minor histocompatibility (H) Ags. Insufficient presentation of nondominant epitopes in the presence of dominant epitopes was investigated as a possible mechanism for immunodominance. Ag presentation was assessed...

By selecting phage display libraries with immune sera from experimentally infected pigs, porcine B-cell epitopes in the open reading frame (ORF) 2, 3, 5 and 6 proteins of European-type porcine reproductive and respiratory syndrome virus (PRRSV) were identified. The sequences of all the epitopes were well conserved in European-type PRRSV and even between European- and American-type PRRSV. Accord...

The human cytotoxic T-lymphocyte (CTL) response to human immunodeficiency virus type 1 (HIV-1) has been intensely studied, and hundreds of CTL epitopes have been experimentally defined, published, and compiled in the HIV Molecular Immunology Database. Maps of CTL epitopes on HIV-1 protein sequences reveal that defined epitopes tend to cluster. Here we integrate the global sequence and immunolog...

The identification of T cell epitopes is crucial for the understanding of the host immune response during infection. While much is known about the MHC class I-restricted response following influenza virus infection of C57BL/6 mice, with over 16 CD8 epitopes identified to date, less is known about the MHC class II-restricted response. Currently, only a few I-A(b)-restricted T helper epitopes hav...

Brucellosis is one of the major zoonoses in the word that widely inflicts health problems in animal and human. In China, brucellosis is gradually increasing in recent years, especially in the xinjiang. Therefore, this study is to predict Brucella T-and B-combined epitopes of OMP31 protein by bioinformatics software. B-cell epitopes of OMP31 protein is predicted using DNAStar and IEDB software. ...

A series of potently neutralizing monoclonal antibodies (MAbs) that target quaternary epitopes on the native Env trimer have recently been described. A common feature shared by these antibodies is the critical involvement of sites in both the V2 and V3 variable domains in antibody recognition. In this study the gp120 variable-region determinants were mapped for eight rhesus macaque monoclonal a...

It is commonly assumed that antibody responses against the influenza virus are polarized in the following manner: strong antibody responses are directed at highly variable antigenic epitopes, which consequently undergo 'antigenic drift', while weak antibody responses develop against conserved epitopes. As the highly variable epitopes are in a constant state of flux, current antibody-based vacci...