Three rapid assay techniques (latex agglutination, laser nephelometry, and EMIT enzyme immunoassay) have been evaluated for serial monitoring of the serum C reactive protein (CRP) concentration in immunosuppressed patients with fever. Radial immunodiffusion assay was used as a reference method. Latex agglutination reliably distinguished between normal and raised serum CRP concentrations. Enzyme...

An enzyme immunoassay technique based on the direct detection of Chlamydia trachomatis antigen in urethral or cervical swabs was used for the rapid diagnosis of chlamydial genital infection. Urethral and cervical samples from 140 patients were tested in parallel by enzyme immunoassay and cell culture using iodine staining. The direct test had a sensitivity of 92.5% and specificity of 97.2% when...

We report an evaluation of the performance characteristics, accuracy, and sensitivity of five test kits designed to detect human choriogonadotropin (hCG) in urine: "Sensitex" (latex agglutination inhibition), "beta-Neocept" (hemagglutination inhibition), "Duoclone" (direct agglutination), "Tandem-Visual HCG" (enzyme immunoassay), and "Sensichrome" (enzyme immunoassay). For comparison, all speci...

We have realized a cloned enzyme donor immunoassay (CEDIA) on a microchip in 96 s. CEDIA is a homogeneous immunoassay, based on the bacterial enzyme beta-galactosidase, which was genetically engineered into two inactive fragments: an enzyme donor and an enzyme acceptor. A model analyte was theophylline, and the detectable concentration range was from 0 to 40 microg mL(-1). Our CEDIA using a mic...

Vol. 26, No. 12, 1980 3. Jaklitsch, A. P., Schneider, R. S., Johannes, R. J., et aL, Homogeneous enzyme immunoassay for T-4 in serum. C/in. Chem. 22,1185 (1976). Abstract. 4. Galen, R. S., and Forman, D., Enzyme immunoassay of serum thyroxine with the “Auto Chemist” multichannel analyzer. C/in. Chem. 23, 119-121 (1977). 5. Finley, P. R., and Williams, R. J., Evaluation of homogeneous enzyme imm...

Introduction: The diagnosis of pregnancy from forensic bloodstains can be useful in cases infanticide, criminal abortions and missing person identification. Objective: This research illustrated the use a rapid, precise, tremendously responsive enzyme immunoassay kit designed for medical usage, which is put to good our lab qualitative HCG detection blood stains has proven tool Methods: concentra...