A chemoenzymatic method for the synthesis of haloethers is presented. combination enzymatic hypohalite with spontaneous oxidation alkenes and nucleophilic attack by various alcohols enabled a wide range haloethers. The reaction system has been characterised current imitations have worked out. In present, aqueous system, hydroxyhalide formation represents main undesired side reaction. Neverthele...

MOTIVATION The metabolic pathway is an important biochemical reaction network involving enzymatic reactions among chemical compounds. However, it is assumed that a large number of metabolic pathways remain unknown, and many reactions are still missing even in known pathways. Therefore, the most important challenge in metabolomics is the automated de novo reconstruction of metabolic pathways, wh...

Recent interest in modeling biochemical networks raises questions about the relationship between often complex mathematical models and familiar arithmetic concepts from classical enzymology, and also about connections between modeling and experimental data. This review addresses both topics by familiarizing readers with key concepts (and terminology) in the construction, validation, and applica...

The enzymatic kinetic resolution of tert-butyl 2-(1-hydroxyethyl) phenylcarbamate via lipase-catalyzed transesterification reaction was studied. We investigated several reaction conditions and the carbamate was resolved by Candida antarctica lipase B (CAL-B), leading to the optically pure (R)- and (S)-enantiomers. The enzymatic process showed excellent enantioselectivity (E > 200). (R)- and (S)...

We obtain lower and upper bounds on the skewness and kurtosis associated with the cycle completion time of unicyclic enzymatic reaction schemes. Analogous to a well known lower bound on the randomness parameter, the lower bounds on skewness and kurtosis are related to the number of intermediate states in the underlying chemical reaction network. Our results demonstrate that evaluating these hig...

We obtain lower and upper bounds on the skewness and kurtosis associated with the cycle completion time of unicyclic enzymatic reaction schemes. Analogous to a well-known lower bound on the randomness parameter, the lower bounds on skewness and kurtosis are related to the number of intermediate states in the underlying chemical reaction network. Our results demonstrate that evaluating these hig...

We present a new type of allosteric modulation in which a molecule bound outside the active site modifies the chemistry of an enzymatic reaction through rapid protein dynamics. As a test case for this type of allostery, we chose an enzyme with a well-characterized rate-promoting vibration, lactate dehydrogenase; identified a suitable small molecule for binding; and used transition path sampling...

The aim of this study was to investigate the effects of different acid catalysts and pretreatment factors on the hydrolysis of biomass compounds over a range of thermochemical pretreatments; maleic, oxalic, and sulfuric acids were each used under different pretreatment conditions. The most influential factor for fermentable sugar production in the dicarboxylic acid-pretreated mixed hardwood was...

The size of the gel preparation, the concentration of the encapsulated enzyme and the ratio of the preparation volume to the volume of the reaction mixture influence the reaction efficiency with encapsulated biocatalysts. A model of first order enzymatic reaction with substrate diffusion is presented and validated by the decomposition reaction of hydrogen peroxide by catalase. The Thiele modulu...

An enzymatic assay system for nitrate employing the membrane-bound nitrate reductase (EC 1.7.99.4) of E. coli is described. Contrary to previous enzymatic assay systems, the present method is a kinetic one, i.e. the substrate, nitrate, is assayed by measuring the reaction rate of the nitrate reductase-catalyzed reaction. Based on the observation that the nitrate reductase-catalyzed reaction obe...