• Conversion feasibility of bread waste (BW) into bioethanol and biomethane. High glucose yield (75–98 g/L) was achieved from acidic/enzymatic hydrolysis BW. Ethanol 0.47 g/g AH BMP 345 CH 4 /g VS ABW + FR obtained. 0.49 EH 379 EBW BW-based ethanol reduces 50% fossil energy use GWP 0.018 kg CO 2 /kg Bread is the second most wasted food in UK with annual wastage 292,000 tons. In present work, ut...

Background The crystallinity of cellulose is a principal factor limiting the efficient hydrolysis of biomass to fermentable sugars or direct catalytic conversion to biofuel components. We evaluated the impact of TFA-induced gelatinization of crystalline cellulose on enhancement of enzymatic digestion and catalytic conversion to biofuel substrates. Results Low-temperature swelling of cotton li...

Clostridial fermentation of cellulose and hemicellulose relies on the cellular physiology controlling the metabolism of the cellulosic hexose sugar (glucose) with respect to the hemicellulosic pentose sugars (xylose and arabinose) and the hemicellulosic hexose sugars (galactose and mannose). Here, liquid chromatography-mass spectrometry and stable isotope tracers in Clostridium acetobutylicum w...

The limitation of surface-display systems in biofuel cells to a single redox enzyme is a major drawback of hybrid biofuel cells, resulting in a low copy-number of enzymes per yeast cell and a limitation in displaying enzymatic cascades. Here we present the electrosome, a novel surface-display system based on the specific interaction between the cellulosomal scaffoldin protein and a cascade of r...