The zona pellucida (ZP) is an extracellular membrane surrounding mammalian oocytes. The so-called zona hardening plays a key role in fertilization process, as it blocks polyspermy, which may also be caused by an increase in the mechanical stiffness of the ZP membrane. However, structural reorganization mechanisms leading to ZP's biomechanical hardening are not fully understood yet. Furthermore,...

Most epidemiologic studies on bovine leptospirosis are based on serological tests that use antibodies against several serotypes, including the serovar Hardjo, which is widespread and considered to be the most adapted to bovine hosts. However, using only serological studies is not sufficient to identify and distinguish species of leptospires. The aim of this study was report the first isolation ...

Bovine mastitis is an inflammation of the udder caused by microbial infection. Mastitis caused by Staphylococcus aureus is a major concern to the dairy industry due to its resistance to antibiotic treatment and its propensity to recur chronically. Growing concerns surrounding antibiotic resistance have spurred research into alternative treatment methods. The ability of lytic S. aureus bacteriop...

Leptospirosis is a common disease of livestock, pet many countries for cattle, dogs and swine but vaccination may animals and wildlife throughout the world. Sporadic cases and prove only partially effective due to generation of serovar outbreaks of the disease have been reported from USA, U.K, specific immunity, prevalence of several Leptospira serovars Australia, New Zealand, USSR and countrie...

Seventy-two Pasteurella multocida strains collected from calves with bronchopneumonia were examined. An analysis of the polymorphism of DNA restrictive fragments with the use of the pulsed-field gel electrophoresis technique of contour-clamped homogeneous electric field electrophoresis was made. Philogenetic relatedness among the examined strains was calculated by the unweighted pair-group meth...

Various aspects associated with anergy to tuberculin were investigated in a total of 75 tuberculous cattle. Forty-six (59%) had single lesions and 32 (41%) had multiple lesions. Forty-three (55.1%) had lesions in the lungs or associated lymph nodes and 23 (29.5%) showed multiple involvement of these tissues. Nine anergic cattle had generalised tuberculosis. Histological examination of lesions f...

The recombinant leptospiral protein LipL32 was evaluated for use in the diagnosis of bovine leptospirosis by enzyme-linked immunosorbent assay (rLipL32 IgG ELISA). The microscopic agglutination test (MAT) of 150 serum samples from cattle suspected of leptospirosis showed that 125 (83.3%) samples had positive reciprocal agglutination titres, which ranged from 100 to 1600. The highest titres were...

Background: The enzootic bovine leukosis (EBL) is a viral disease with chronic aspect and its etiological agent an oncogenic deltaretrovirus called leukemia virus (VLB). It has wide distribution in dairy cattle herds, it may take several years before the first clinical manifestations occur. Animals seropositive for VLB are more susceptible to developing infectious diseases such as mastitis. obj...