background: we examined a molecular method with a single-pcr for amplification of a part of cp5 gene enabling us to differen­tiate the pathogenic species, entamoeba histolytica , from the non-pathogenic species, e. dispar . methods: we developed a single pcr method for this purpose.  after investigation of genbank, primer pairs were de­signed from highly conserved regions of cysteine proteinase...

The aim of this study was to determine the prevalence of amoebic infection in non-human primates (NHPs) from six Zoological gardens in the United Kingdom. Initially, 126 faecal samples were collected from 37 individually identified NHPs at Twycross Zoo, UK, and were subjected to microscopic examination. A subsequent, nationwide experiment included 350 faecal samples from 89 individually identif...

The protozoan parasite Entamoeba histolytica is responsible for invasive intestinal and extraintestinal amebiasis. The virulence of Entamoeba histolytica is strongly correlated with the parasite's capacity to effectively kill and phagocytose host cells. The process by which host cells are killed and phagocytosed follows a sequential model of adherence, cell killing, initiation of phagocytosis, ...

BACKGROUND The infectious and diagnostic form of Entamoeba histolytica (Eh), cause of amebic dysentery and liver abscess, is the quadranucleate cyst. The cyst wall of Entamoeba invadens (Ei), a model for Eh, is composed of chitin fibrils and three sets of chitin-binding lectins that cross-link chitin fibrils (multivalent Jacob lectins), self-aggregate (Jessie lectins), and remodel chitin (chiti...

Lateral gene transfer (LGT) has impacted the evolutionary history of eukaryotes, though to a lesser extent than in bacteria and archaea. Detecting LGT and distinguishing it from single gene tree artifacts is difficult, particularly when considering very ancient events (i.e., over hundreds of millions of years). Here, we use two independent lines of evidence--a taxon-rich phylogenetic approach a...

Entamoeba histolytica cells, the cause of amoebic dysentery, are highly motile, and this motility is an essential feature of the pathogenesis and morbidity of amoebiasis. However, the control of E. histolytica motility within the gut and during invasion is poorly understood. We have used an improved chemotaxis assay to identify the key extracellular signals mediating Entamoeba chemotaxis. The d...

Clostridial glucosylating cytotoxins inactivate mammalian Rho GTPases by mono-O glucosylation of a conserved threonine residue located in the switch 1 region of the target protein. Here we report that EhRho1, a RhoA-like GTPase from the protozoan parasite Entamoeba histolytica, is glucosylated by clostridial cytotoxins. Recombinant glutathione S-transferase-EhRho1 and EhRho1 from cell lysate of...

Specimen 1 Organisms Referees Frequency Ext 1 Ext 2 571 Ascaris lumbricoides 18 Moderate to Many 15 49 545 Entamoeba coli 10 Moderate to Many 4 16 589 Schistosoma mansoni 6 Few to moderate 3 11 524 Parasite(s) found referred for ID 10 580 Trichuris trichiura 3 Few to moderate 6 544 Endolimax nana 2 2 574 Hookworm 1 546 Entamoeba hartmanni 1 548 Entamoeba histolytica / Entamoeba dispar 1 566 Hym...