background: the values of embryonic stem cell and cloning are evident. production of clone from embryonic stem cells can be achieved by introduction of stem cell into a tetraploid blastocyst. tetraploid blastocyst can be produced in vitro by electrofusion of 2-cell embryos. objective: the aim of this study was to assess the effect of different voltages and durations on fusion rate of bovine 2-c...

Induced pluripotent stem cells (iPSCs) are reprogrammed from somatic cells through numerous transcription factors. Human induced pluripotent stem cell approaches are developing as a hopeful strategy to improve our knowledge of genetic association studies and the underlying molecular mechanisms.  Rapid progression in stem cell therapy and cell reprogramming provides compelling reasons for its fe...

Objective(s): In the past few decades, variety of foetal, embryonic and adult stem and progenitor cells have been tried with conflicting outcome for cell therapy of central nervous system injury and diseases. Cellular characteristics and functional plasticity of Globose basal stem cells (GBCs) residing in the olfactory epithelium of rat olfactory mucosa have not been studied in the past by the ...

To find a suitable feeder layer is important for successful culture conditions of bovine embryonic stem cell-like cells. In this study, expression of pluripotency-related genes OCT4, SOX2 and NANOG in bovine embryonic stem cell-like cells on mouse embryonic fibroblast feeder layers at 1-5 passages were monitored in order to identify the possible reason that bovine embryonic stem cell-like cells...

introduction: stem cell biology has been the subject of much recent discussion. embryonic stem (es) cells, derived from the inner cell mass of the blastocyst stage of early mammalian embryos are expected to become a powerful tool in future regenerative medicine and developmental biology due to their capacity of self-renewal and pluripotency. in the present study, the ultrastructural development...

background: human cripto-1, a member of the egf-cfc family, is involved in embryonic development, embryonic stem cell maintenance, and tumor progression. it also participates in multiple cell signaling pathways including wnt, notch, and tgf-β. remarkably, it is expressed in cancer stem cell (csc) compartments, boosting tumor cell migration, invasion, and angiogenesis. although cripto-1 is overe...

Background: Human Cripto-1, a member of the EGF-CFC family, is involved in embryonic development, embryonic stem cell maintenance, and tumor progression. It also participates in multiple cell signaling pathways including Wnt, Notch, and TGF-β. Remarkably, it is expressed in cancer stem cell (CSC) compartments, boosting tumor cell migration, invasion, and angiogenesis. Although Cripto-1 is ...

Blastocyst injection and morula aggregation are commonly used to evaluate stem cell pluripotency based on chimeric contribution of the stem cells. To assess the protocols for generating chimeras from stem cells, 8-cell mouse embryos were either injected or cocultured with mouse embryonic stem cells and induced pluripotent stem cells, respectively. Although a significantly higher chimera rate re...

Embryonic Hematopoiesis In article number 2205345, Fudi Wang, Junxia Min, and co-workers identify demonstrate indispensable function of zinc transporter SLC39A10 (ZIP10) its mediated homeostasis for maintaining the survival fetal hematopoietic stem/progenitor cells. As most important importer on cell membrane stem cells, ZIP10 functions as a gatekeeper (like vacuum to transport across membrane)...

embryonic stem cells (escs) as pluripotent stem cells have the potential to give rise to all three germ layer cells. also, these cells have the self-renewing ability. escs could differentiate into most of cell lineages. lymphocytes generated from hematopoietic stem cells. we designed a feeder free culture condition to generate lymphoid lineage cells from escs. we demonstrated that il-7 plays cr...