background: to compare the pregnancy outcomes after two embryos versus three embryos transfers (ets) in women undergoing in vitro fertilization (ivf)/intracytoplasmic sperm injection (icsi) cycles. materials and methods: this retrospective study was performed on three hundred eighty seven women with primary infertility and with at least one fresh embryo in good quality in order to transfer at e...

Background The purpose was to investigate the effect of the duration of gamete incubation on fertilization rate, embryo cleavage, and embryo quality before and after freezing in mice. MaterialsAndMethods Ovulated oocytes collected from superovulated mice after ip injection of PMSG and hCG were divided randomly into control and experimental groups. Oocytes from the control group were inseminated...

background: embryo transfer (et) has traditionally been performed two days after oocyte retrieval. delaying transfer from day two to day three or four would allow for further development of the embryo might therefore optimize the selection of viable and best quality embryos for transfer and may be closer to the physiological time of the entry into the uterus than transfer on day two and might h...

BACKGROUND Embryos selection is crucial to maintain high performance in terms of pregnancy rate, reducing the risk of multiple pregnancy during IVF. Pronuclear and nucleolar characteristics have been proposed as an indicator of embryo development and chromosomal complement in humans, providing information about embryo viability. METHODS To correlate the zygote-score with the maternal age and ...

OBJECTIVES In vitro maturation technique (IVM) is shown to have an effect on full maturation of immature oocytes and the subsequent embryo development. Embryonic genome activation (EGA) is considered as a crucial and the first process after fertilization. EGA failure leads to embryo arrest and possible implantation failure. This study aimed to determine the role of IVM in EGA-related genes expr...

Embryo transfer offers great advantages to South American camelid farmers to reach their breeding goals but the technology still plays a relatively minor role in comparison to other domestic farm animals like cattle. The aim of the present study was to analyse a data set of 5547 single or multiple ovulation embryo transfers performed in commercial alpaca farms in Australia to determine the fact...

The main purpose for human assisted reproductive technologies (ART) is clearly to bypass reproductive failures. This purpose is totally different from the aims in animal ART, being genetic improvement and more recently conservation of endangered species. Apoptosis or programmed cell death has been detected before implantation in in vivo as well as in vitro embryos and might contribute to lower ...

The inferior oocytes (IOs), which are not suitable for embryo development, occupy roughly one-third or more of the collected immature bovine oocytes. The IOs are usually discarded from the in vitro bovine embryo production process. Improving the quality of the inferior oocytes (IOs) and make them available in in vitro embryo production would have important biological, as well as commercial, val...

BACKGROUND The main reason for adverse treatment outcome in assisted reproduction is the high rate of multiple pregnancies. The only strategy to avoid dizygotic twins is to transfer one embryo at a time. METHODS A total of 144 women, who had had at least four good quality embryos available after IVF/intracytoplasmic sperm injection (ICSI) and who had no more than one previous failed treatment...

BACKGROUND Single embryo transfer (SET) after IVF/ICSI has been shown to result in an acceptable pregnancy rate in selected subjects. In our unit, SET is routinely carried out among women under the age of 36 in the first or second treatment cycle when a top-quality embryo is available. In order to define further the selection criteria for SET, we have analysed the outcome of elective SET (eSET)...