An assay for immune complexes [ICs] in gingival crevicular fluid [GCF] could be potentially useful to ascertain the risk of periodontal disease activity at specific oral sites. This paper describes and ELISA-based method sufficiently sensitive to measure ICs in GCF samples at levels below 1 microgram/ml. Static GCF samples taken from 5 adults showed IC levels ranging from 5 to 166 micrograms/ml...

Polyclonal antibodies were produced to detect the coccidiostat nicarbazin. Due to structural constraints of the active component of nicarbazin, dinitrocarbanilide (DNC), three different compounds that shared a common substructure with DNC were used as antigen mimics. The compounds (N-succinyl-L-alanyl-L-alanyl-L-alanine 4-nitroanilide (SAN), L-glutamic acid gamma-(p-nitroanilide) (GAN) and p-ni...

To explore whether antibodies against beta2-glycoprotein I (beta2GPI) complexed to 7-ketocholesteryl-9-carboxynonanoate (oxLig-1) and to oxidised low-density lipoproteins (oxLDL) relate to paraoxonase activity (PONa) and/or intima media thickness (IMT) of carotid arteries in primary antiphospholipid syndrome (PAPS). As many as 29 thrombotic patients with PAPS, 10 subjects with idiopathic antiph...

abstract: potato virus y a type species of the genus potyvirus infects several crops in the family solanaceae. pvy isolated from field infected peppers was identified on the basis of host reaction, serological and molecular characterization. the result of elisa, immunoblot electrophoresis and immuno capture reverse transcription polymerase chain reaction   (ic-rt-pcr) indicated that pepper isol...

fanleaf degeneration, a disease caused by grapevine fanleaf virus (gflv), is considered as the most destructive grapevine viral disease worldwide causing severe losses in vineyards, annually. gflv is apparently believed to have originated from ancient persia and therefore, to restrict it in its further infections, a precise identification along with a monitoring of its distribution pattern is c...

porous media has interesting features in compared with free flame combustion due to the extended of the lean flammability limits and lower emissions. advanced new generation of internal combustion (ic) engines are expected to have far better emissions levels both gaseous and particulate matter, at the same time having far lower fuel consumption on a wide range of operating condition. these crit...

Serology is based on the use of antibodies to detect circulating antibodies (immunoglobulin) or antigens. Largely used in the diagnosis of infectious diseases, it is now often replaced by DNA or RNA detection of infectious agents by molecular biology techniques such as polymerase chain reaction (PCR, rt-PCR). However, antibody detection can have some advantages on PCR techniques and numerous di...