OBJECTIVE The aim of this study was to analyze the influence of autoantibodies against spermatozoa present in the semen on the outcome of in vitro fertilization with intracytoplasmic sperm injection (ICSI). MATERIALS AND METHODS We performed a retrospective analysis of clinical and laboratorial data from a six year-period ICSI cycles. Screening for the presence of ASA in the semen, by using t...

For artificial insemination (AI), boar semen is prepared in rather simple extenders, kept at 16-20°C and usually used in liquid form for up to 3 days from extension. Routine freezing of boar semen is occasionally used for gene banking, replacement of lost genetics or long-distance transport [1]. The reason behind this meagre use of freezing is the low tolerance of the ejaculated boar sperm to c...

epididymal spermatozoa. The biological activity of the extracts seemed to result, at least in part, from their ability to prevent energy utilization by uncoupling oxidative phosphorylation (8, 11). More recently, it has been possible to separate the yeast fermentation stimulating activity from that which causes uncoupling of oxidative phosphory lation in rat kidney or liver preparations. In fre...

this study was aimed to evaluate the effect of gnrh injection on semen ejaculate characteristics in awassi rams collected in breeding and non breeding seasons. the study was conducted in late summer to early autumn when breeding activity commences, and in winter during the non breeding season. twelve mature awassi rams were used in this study. animals were randomly allocated into two equal grou...

The roles ofselenium (Se) and glutathione peroxidase in reproductive function are poorly understood, but it is possible that they may be important for normal reproduction in the male. In rats fed a Se-deficient diet, the testes accumulated and retained more 75Se than did other tissues 1 week after injection (Brown & Burk, 1972; Burk, Brown, Seely & Scaief, 1972). Autoradiographic studies (Brown...

This study was designed to determine the degree and type of bacterial contamination of ejaculated semen samples in fertile rams and its consequences on sperm quality during storage. In experiment 1, 68 ejaculates from 36 rams were divided into two aliquots, one of which was used for bacterial culture, while the other one was diluted, stored at 15°C and assessed for plasma membrane integrity and...

Certain factors may affect sperm motility and integrity in a freezing process. Reactive Oxygen Species (ROS) is essential damaging the sperm, as previous study revealed that ROS major cause membrane of spermatozoa. This aimed to understand production its interaction within motility, mitochondrial activity DNA damage cryopreservation process Indonesian Ongole grade bull. A total 40 ejaculated se...

tau.amegroups.com © Translational Andrology and Urology. All rights reserved. We read with interest the commentary by Dr. Paul Turek (1) contextualizing the use of sperm DNA fragmentation (SDF) testing for male infertility in response to the recently published practice recommendations for SDF testing based on clinical scenarios by Agarwal et al. (2). We certainly concur with the author regardin...

This report deals with some gross characteristics of baboon (Papio sp.) semen ejaculated spontaneously or collected by electro-ejaculation. Details of the equipment used and the collection procedure are described and the results of freezing trials are presented. The animals were sedated with Sernylan (Parke, Davis and Co.) at a dose of 0\m=.\8mg/kg intramuscularly. They were then tied in an ext...

BACKGROUND Features of both apoptosis and necrosis have been reported in ejaculated human spermatozoa. This study examines the relative contribution of these two modes of cell death to the demise of these terminally differentiated cells. METHODS Sperm fractions were prepared from aliquots of semen samples from young normozoospermic donors by simple washing from seminal plasma, by discontinuou...