Synthetic prions were produced in our laboratory by using recombinant mouse prion protein (MoPrP) composed of residues 89-230. The first mouse synthetic prion strain (MoSP1) was inoculated into transgenic (Tg) 9949 mice expressing N-terminally truncated MoPrP(Delta23-88) and WT FVB mice expressing full-length MoPrP. On first and second passage in Tg9949 mice, MoSP1 prions caused disease in 516 ...

To understand the role of the cell cycle regulatory protein in the control of smooth muscle cell (SMC) proliferation, we tested the overexpression of p21Waf1, a cyclin-dependent kinase inhibitor, in human normal (MS9) and immortalized SMCs (ISS10) transfected with ori-minus simian virus 40 DNA, using an adenovirus-mediated system. In MS9, overexpression of p21Waf1 resulted in the inhibition of ...

Avian leukosis (AL) is one of the most harmful diseases to the poultry industry in China. The detection of the avian leukosis virus (ALV) p27 antigen plays a decisive role in the elimination of avian leukosis. To explore the influence of passaging cells on the detection rate of the ALV p27 antigen, 21 aseptic anticoagulated blood samples were collected from 21 chickens for which the cloacal swa...

To isolate stem-like cells from the human MG-63 osteosarcoma cell line, different subpopulations of MG-63 cells were cloned by limiting dilution and passaged to obtain different sublines. The subline with highest clonogenicity was identified using a proliferation assay, cell-cycle analysis, and soft-agar colony-forming assay. The sublines were further selected in serum-free medium containing 20...

The effect of sodium and magnesium chloride in 1 and 2 m concentration at temperatures of 37 and 50 C on type C, strain 149, foot-and-mouth disease virus during storage for 6 days was studied. The exclusively passaged cattle strain and its tissue culture-adapted line were compared. Preparations of the various chemicals and their concentrations were made directly in suspensions of the virus, whi...

the present research was conducted to analyze the genetic structure of egg quality traits in japanese quail. external and internal egg quality traits were assessed on 1706 eggs of laying japanese quail. eggs were collected for 4 days at the quaits 10 weeks of age. the investigated traits were analyzed through univariate as well as multivariate animal models employing restricted maximum likeliho...

The study was conducted to determine the effect of varying protein levels and preservation method on external egg qualities of Japanese quails. A total of 180 birds were randomly allotted into three dietary treatments in a completely randomized design (CRD) replicated six times. A factorial arrangement of 3 × 4 × 4 which involves 3 protein levels (15, 20 and 25% crude protein), 4 storage method...

the present research was designed to examine the genetic analysis of egg weight, egg yolk weight and egg albumen weight of bovan neva black laying hens at 25, 51 and 72 weeks. for this purpose, thirty birds were selected from the layer flock in the babcock university teaching and research farm. they were individually housed in separate labeled battery cage. a total of thirty eggs were collected...