The time taken to achieve peak tidal expiratory flow as a proportion of total expiratory time (t PTEF/t E) during tidal breathing (TB) is used as a parameter of airway obstruction in children with asthma. Curve selection bias is one of the most important limitations to the method. This study evaluates three curve selection methods, including a computer program, which on-line selects and analyse...

Antibiotic resistance genes and antibiotics are frequently used to maintain plasmid vectors in bacterial hosts such as Escherichia coli. Due to the risk of spread of antibiotic resistance, the regulatory authorities discourage the use of antibiotic resistance genes/antibiotics for the maintenance of plasmid vectors in certain biotechnology applications. Overexpression of E. coli endogenous fabI...

We consider the problem of dynamically cross-selling products (e.g., books) or services (e.g., travel reservations) in the e-commerce setting. In particular, we look at a company that faces a stream of stochastic customer arrivals and may offer each customer a choice between the requested product and a package containing the requested product as well as another product, what we call a “packagin...

Multiple Reaction Monitoring (MRM), also known as Selected Reaction Monitoring (SRM), is a powerful targeted proteomics method. The approach is based on the generation of specific quantitative assays for each protein of interest and can be used to accurately quantitate large sets of proteins at high throughput. One challenge in Multiple Reaction Monitoring Proteomics analysis is however to sele...

Building collaborative relationships with trusted businesses is a longterm strategy for EU governments. Recently, for the EU Tax and Customs Administration (TCA), the realization of this goal has become more visible with the emerging concept of the Authorized Economic Operator (AEO). Businesses in the member states can apply for the AEO certificate. When it is being granted, simplified control ...

The selection of highly discriminatory features has been crucial in aiding further advancements in domains such as biomedical sciences, high-energy physics and e-commerce. Therefore evaluation of the robustness of feature selection methods to small perturbations in the data, known as feature selection stability, is of great importance to people in these respective fields. However, little resear...

Department of Experimental Oncology, European Institute of Oncology, 20141 Milan, Italy [P. G. P., L Li; Istituto di Medicine Interna e Scienze Oncologiche, Perugia University, 06100 Perugia, Italy (Fr. G., A. M., D. R., Fa. G., P. G. P.]; Hematology Oncology Laboratory. Istituto Superiore di Sanità 00161 Rome, Italy (Fr. G., M. V., C. P.]; Department of Molecular Pharmacology, Stanford Univers...

Maximum likelihood and Bayesian approaches are presented for analyzing hierarchical statistical models of natural selection operating on DNA polymorphism within a panmictic population. For analyzing Bayesian models, we present Markov chain Monte-Carlo (MCMC) methods for sampling from the joint posterior distribution of parameters. For frequentist analysis, an Expectation-Maximization (EM) algor...

An e-learning architecture selection has been analyzed. This is a typical problem when dealing with e-learning system selection. For each alternative of an e-learning architecture there is an evaluation of both cost and quality of service. The latter may include probabilistic delivery time and confidence in quality commitment. The decision-maker takes into account multi-criteria evaluation thro...

The aspartic protease cathepsin E has been shown to induce apoptosis in cancer cells under physiological conditions. Therefore, cathepsin E-activity-enhancing peptides functioning in the physiological pH range are valuable potential cancer therapeutic candidates. Here, we have used a general in vitro selection method (evolutionary rapid panning analysis system (eRAPANSY)), based on inverse subs...