Abstract A microarray detection method based on on-chip signal amplification using terminal deoxynucleotidyl transferase (TdT) was developed to visualize pathogenic genes. Cyclic olefin copolymer (COC) substrate for microarrays treated with oxygen plasma induce hydrophilic surface properties. The capture probe DNA immobilized the COC by UV irradiation. 3ʹ end of modified a phosphate group provi...