Biomineralization is an important process, which is often assisted by biomolecules. In this paper, the effect of chondroitin sulfate on the crystallization of hydroxyapatite was examined quantitatively based on a generic heterogeneous nucleation model. It is found that chondroitin sulfate can suppress the supersaturation-driven interfacial structure mismatch between the hydroxyapatite crystal a...

1 Institute for Applied Physics, NAS of Ukraine, 58, Petropavlovskaya Str., 40000 Sumy, Ukraine 2 Lanzhou Institute of Chemical Physics, 18, Tianshui Middle Road, 730000 Lanzhou, Gansu, China 3 Key Laboratory of Synthetic and Natural Functional Molecular Chemistry of the Ministry of Education, College of Chemistry and Materials Science, Northwest University, 229 North Taibai Road, 710069 Xi’an,...

Hydroxyapatite (HA) has been used for IgG purification since its introduction in the 1950s. Applications expanded to include IgA and IgM in the 1980s, along with elucidation of its primary binding mechanisms and the development of ceramic HA media. With the advent of recombinant monoclonal antibodies, HA was demonstrated to be effective for removal of antibody aggregates, as well as host cell p...

Two distinct tRNA (m G) methylases have been found in the yeast Saccharomyces cerevisiae. They differ in their chromatographic properties on hydroxyapatite, in their response to spermine, and in their site specificity. Only one of the methylases is active against normal tRNA from Escherichia col

Diffusion-controlled dissolution and precipitation reactions occur in many biological systems and some non-stirred in vitro systems. Previous studies have shown that differences in the diffusion rates of the ions involved in a dissolution/precipitation reaction can produce significant effects on the rate and course of the reaction. We report here results of a study that show inter-diffusion of ...

Figure 1: (a) Images of hydroxyapatite (HA) coated Mg (Left) and uncoated Mg (Right) for degradation study. (b) Degradation of HA coated Mg (Left) compared to uncoated Mg (Right) after 12 days of incubation in PBS. Uncoated Mg showed larger area of degradation and more degradation products (white particles surround the sample). (c,d) SEM image and EDX analysis of HA coated Mg after 12 days of d...

a Interdisciplinary Nanoscience Center (iNANO), Aarhus University, 8000 Aarhus C, Denmark b Department of Physics and Astronomy, Aarhus University, 8000 Aarhus C, Denmark c Orthopaedic Research Laboratory, Aarhus University Hospital, 8000 Aarhus C, Denmark d Department of Industrial and Environmental Biotechnology, National Institute of Genetic Engineering and Biotechnology, P.O. Box: 14965/161...

The adsorption of human serum albumin onto hydroxyapatite-modified silver electrodes has been in situ investigated by utilizing the piezoelectric quartz crystal impedance technique. The changes of equivalent circuit parameters were used to interpret the adsorption process. A kinetic model of two consecutive steps was derived to describe the process and compared with a first-order kinetic model ...

Fracture mechanisms of an enamel-like hydroxyapatite-collagen composite model are elaborated by means of molecular and coarse-grained dynamics simulation. Using fully atomistic models, we uncover molecular-scale plastic deformation and fracture processes initiated at the organic-inorganic interface. Furthermore, coarse-grained models are developed to investigate fracture patterns at the μm-scal...

Protein A and C, which are major components of the acidic proline-rich proteins in human saliva, were digested, before or after adsorption to hydroxyapatite, with alkaline phosphatase, trypsin, thermolysin and a proteinase preparation from salivary sediment. The results demonstrate that the binding site is located in the proline-poor N-terminal part of the protein, possibly between residues 3 ...