A new membrane-permeant DNA stain, SYBR-14, was used in combination with propidium iodide (PI) to estimate the proportion of living sperm in bovine semen. The SYBR-14 stained living sperm while PI only stained degenerate cells that had lost their membrane integrity. Staining with SYBR-14 resulted in the nuclei of living sperm fluorescing bright green. Aliquots containing nearly all living bovin...

Among head and neck Squamous cell carcinomas (HNSCCs), the larynx is one of the sites most involved, being traditionally associated with tobacco and alcohol use, especially when in combination [1]. As squamous carcinoma develops throughout a step progression in various stages of dysplasia, many molecular changes have been described [2]. The implication of Human Papilloma Virus (HPV) in HNSCC de...

Objective: Anticancer activities of Schiff bases S1 and S2 against MG-63 human osteosarcoma cells were performed described using an in vitro evaluation employing cytotoxicity apoptosis assay. Methods: used MTT assay to examine the effect compound on cell viability (S1 S2). Cell morphologies IC50 values obtained. The acridine orange (AO)/ethidium bromide (EB) dual staining technique was determin...

Cases of acute leukemia showing both terminal deoxynucleotidyl transferase (TdT) and myeloperoxidase (MPO) activities are usually classified as acute myelogenous leukemia (AMI). Although some reports have implied overlap of TdT and MPO based on population percentages. direct evidence for simultaneous expression of TdT and MPO by a leukemic blast is lacking. By use of a simple new technique deve...

Corneal ulcer, which is brought on by a breach in the epithelial barrier, dangerous infection of avascular corneal stroma. New treatment strategies are needed, suppressing aggressive nature disease and including combination different drugs. In this study, vancomycin (VAN) fluconazole (FLU) dual-drug loaded dual-layered polyvinyl alcohol gelatin (PVA/GEL) nanofibrous patches produced electrospin...

The identification of microorganisms by flow cytometry was evaluated by using a double staining technique with propidium iodide and fluorescein isothiocyanate and a two dimensional analysis. A diverse group of 19 different species and strains of microorganisms was tested to determine if they could be differentiated by flow cytometry. The organisms tested displayed characteristic and distinct tw...

The role of clusterin in tumor growth and progression remains unclear. Overexpression of cytoplasmic clusterin has been studied in aggressive colon tumors; however, no correlation between clusterin expression and survival in colorectal cancer has been identified to date. We assessed levels of clusterin expression in a group of stage II colorectal cancer patients to assess its utility as a progn...

Abstract Cell viability is one of the critical quality attributes (CQAs) for analyzing and characterizing cellular therapeutic products. The characterization cell essential to ensure quality, safety efficacy during gene therapy development. In this work, we characterized compared two commonly used dual-staining methods, acridine orange/propidium iodide (AO/PI) orange/4′,6-diamidino-2-phenylindo...

Real-time on-site histopathology review of biopsy tissues at the point-of-procedure has great potential for significant clinical value and improved patient care. For instance, on-site review can aid in rapid screening of diagnostic biopsies to reduce false-negative results, or in quantitative assessment of biospecimen quality to increase the efficacy of downstream laboratory and histopathology ...

Background and Aims: Angiogenesis is an important pathological process during progression of plaque formation, which can result in plaque hemorrhage and vulnerability. This study aims to explore non-invasive imaging of angiogenesis in atherosclerotic plaque through magnetic resonance imaging (MRI) and positron emission tomography (PET) by using GEBP11 peptide targeted magnetic iron oxide nanopa...