A separation methodology to isolate natural uranium from its radioactive daughters: Th, Ra, Bi, Pb, Pa and Po, was developed using an only one anion exchange resin and varying concentrations of HCl solutions. Three types of anion exchange resins were tested and the separation process was followed by gamma and alpha spectrometry. Uranium can be quantitatively isolated from its descendants using ...

Preliminary work by paper and thinlayer chromatography have indicated the existence of a number of similar antagonists with different specific activity. In the earlier stage of the purification, it was studied whether the minor components were real or artifacts. We found it convenient to use buffered resin chromatography according to the procedure of Moore et al.2) for the separation of detoxin...

Solid-phase spectrophotometry has been applied to analysis for trace amounts of vanadium. Vanadium was sorbed in a styrene-divinylbenzene-type anion-exchanger Dowex 1-X8 as a vanadium-4-(2-pyridylazo)resorcinol complex. Resin phase absorbances at 562 and 800 nm were measured directly which allowed the determination of vanadium in the range of 0.1-2.4 ng/ml with a RSD of 1.5%. The method has bee...

Tryptic digestion of the diisopropyl phosphoryl-32P-Scarboxymethyl light chain derivative of human plasmin followed by gel filtration on Sephadex G-75 and G-25 and chromatography on Dowex SOW-X2 has resulted in the isolation of three radioactive peptides. These three peptides, having 31,33, and 34 residues, are all derived from the same segment of the plasmin molecule. Structural determinations...

ment, together with a summary of the remarkable chemical reactivity of this unit. Ion exchange chromatography (Dowex 50Wx8) of the acid hydrolysate (10N hydrochloric acid, reflux for 24 hours) of viomycin afforded two basic amino acids, viomycidine and a small amount of a compound which we have termed viocidic acid. Viomycidine was characterised as its crystalline monohydrochloride m. p. 200~20...

A dinucleoside monophosphate was isolated from 5-bromouracil-induced filaments of a thymine auxotroph of Escherichia coli K-12. The dinucleoside monophosphate was fractioned from a ["C]5-bromouracil-labeled perchloric acid extract using Dowex-1-formate ion-exchange chromatography. Sephadex chromatography revealed its molecular weight to be 710. Snake venom phosphodiesterase digest of the dinucl...

A kinetic study of cadmium and lead biosorption to a brown alga, Kjellmaniella crassiforia, was carried out. The shrinking core model derived by M. Gopala Rao and A. K. Gupta (Chem. Eng. J.24, 181, (1982)) was modified and adapted for description of the rate process of cadmium and lead biosorption to the alga. The biosorption rate process was well described and average apparent diffusion coeffi...

The enzyme-substrate complex formed between pyridoxamine-pyruvate transaminase (EC 2.6.1.30) and pyridoxal was reduced with NaBH4. After carboxymethylation and tryptic digestion, pyridoxyl-lysine-containing peptides were isolated by a combination of Sephadex and Dowex 50 chromatography. Analysis of these peptides shows the structure around the pyridoxal-binding lysine residues to be Ala-Asp-Ile...