BACKGROUND Scabies caused by Sarcoptes scabiei is a widespread but a neglected tropical zoonosis. In this study, we characterised a S. scabiei thioredoxin peroxidase (SsTPx) and evaluated a recombinant SsTPx as a diagnostic antigen in rabbits. METHODS The open reading frame of the gene encoding SsTPx-2 was amplified and the recombinant protein was expressed in Escherichia coli cells and purif...

BACKGROUND Eosinophilic meningitis caused by Parastrongylus cantonensis, the rat lung worm is a major public health problem in Thailand. Humans acquire this parasite by eating raw food containing infective Parastrongyliasis is dificult to make because identification of parasite materials by biopsy or chance finding is rarely possible. OBJECTIVE Develop alternative approaches of Parastrongylus...

Citrus tristeza virus (CTV) is the causal agent of tristeza disease, which is one of the most devastating diseases of citrus crops worldwide. This paper describes a method for the rapid detection and genotyping of naturally spreading CTV isolates. This method uses ELISA or dot-blot immunological tests to detect trees infected with CTV. The reaction wells or membrane spots for which there is a p...

BACKGROUND The sensitivity and selectivity of traditional methods limits ultramicro detection of proteins. Bio-barcode amplification detection methods based on nanotechnology enables ultramicro detection of protein. However, bio-barcode amplification detection depends on the oligonucleotides being fixed on a glass chip. It also requires specialized equipment, which limits its application. We in...

Monoclonal antibodies were raised against cystozoites of Sarcocystis muris and characterised. Twelve monoclonal antibodies reacted in the ELISA, Dot-ELISA and IFAT only with homologous antigen. The other twelve showed cross reactions of various degrees with cystozoites of S. gigantea, S. tenella, S. arieticanis, S. capracanis, S. miescheriana and S. suihominis. Proteins of S. arieticanis, S. te...

ARTICLE INFO ABSTRACT Article history: Received 17 June 2012 Accepted 3 September 2012 Available online 1 November 2012 Plasmids play an important role in the virulence and antibiotic resistance of Salmonella. Salmonella carry plasmids having different size and number. Plasmid profile analysis of single isolates of Salmonella typhimurium and Salmonella enteritidis revealed the presence of 4plas...

Antigen from Yersinia pestis was adsorbed on cellulose acetate discs (0.5 cm of diameter) which were obtained from dialysis membrane by using a paper punch. ELISA for human plague diagnosis was carried out employing this matrix and was capable to detect amount of 1.3 microg of antigen, 3,200 times diluted positive serum using human anti-IgG conjugate diluted 1:4,000. No relevant antigen lixivia...

Infection with Neisseria meningitidis group B has been difficult to detect, partly because this bacterial group's polysaccharide is a weak immunogen. This article describes work carried out to test a new procedure (MB-Dot-ELISA) employing a high-titered horse antiserum for detection of N. meningitidis group B antigens. The study assayed cerebrospinal fluid samples from 585 subjects, 574 with su...

BACKGROUND Diagnosis of leptospirosis by the microscopic agglutination test (MAT) or by culture is confined to specialized laboratories. Although ELISA techniques are more common, they still require laboratory facilities. Rapid Diagnostic Tests (RDTs) can be used for easy point-of-care diagnosis. This study aims to evaluate the diagnostic performance of the RDTs LeptoTek Dri Dot, LeptoTek Later...