نتایج جستجو برای: dnmt3b

تعداد نتایج: 838  

Journal: :JCI insight 2017
Jie Shen Cuicui Wang Daofeng Li Taotao Xu Jason Myers John M Ashton Ting Wang Michael J Zuscik Audrey McAlinden Regis J O'Keefe

Osteoarthritis (OA) is the most common form of arthritis worldwide. It is a complex disease affecting the whole joint but is generally characterized by progressive degradation of articular cartilage. Recent genome-wide association screens have implicated distinct DNA methylation signatures in OA patients. We show that the de novo DNA methyltransferase (Dnmt) 3b, but not Dnmt3a, is present in he...

2016
Vikki Ho Janet E. Ashbury Sherryl Taylor Stephen Vanner Will D. King

Among 272 patients undergoing a screening colonoscopy, DNA methylation of DNMT3B and MTHFR, genes encoding enzymes critical to one-carbon metabolism, was quantified in blood leukocytes using Sequenom EpiTYPER®. DNA methylation was quantified in 66 and 28 CpG sites of DNMT3B and MTHFR respectively, and conceptualized using two approaches. First, measures representing average methylation across a...

Journal: :Molecular human reproduction 2013
Philip C Logan Anna P Ponnampalam Michael Steiner Murray D Mitchell

Progesterone, estrogen and cyclic adenosine monophosphate (cAMP) together regulate the decidualization of human endometrial stromal cells in a time-dependent manner. The role of DNA methylation and the three active DNA methyltransferases (DNMTs) in the regulation of decidualization is gaining interest but the exact role of this epigenetic mechanism during decidualization is largely unknown. We ...

ژورنال: ارمغان دانش 2020

Background and aim: Lung cancer is one of the most leading causes of cancer death in males and females and the second leading cause of cancer death. Epigenetic alterations, including DNA hypermethylation, histone deacetylation, and miRNAs lead to the silencing of tumor suppressor genes (TSGs) resulting in tumorigenesis. This change has been reported in various cancers. The activity of DNA meth...

2014
Kevin Huang Yin Shen Zhigang Xue Marina Bibikova Craig April Zhenshan Liu Linzhao Cheng Andras Nagy Matteo Pellegrini Jian-Bing Fan Guoping Fan

Whether human induced pluripotent stem cells (hiPSCs) are epigenetically identical to human embryonic stem cells (hESCs) has been debated in the stem cell field. In this study, we analyzed DNA methylation patterns in a large number of hiPSCs (n = 114) and hESCs (n = 155), and identified a panel of 82 CpG methylation sites that can distinguish hiPSCs from hESCs with high accuracy. We show that 1...

Journal: :Nature communications 2016
Christopher E Duymich Jessica Charlet Xiaojing Yang Peter A Jones Gangning Liang

Promoter DNA methylation is a key epigenetic mechanism for stable gene silencing, but is correlated with expression when located in gene bodies. Maintenance and de novo DNA methylation by catalytically active DNA methyltransferases (DNMT1 and DNMT3A/B) require accessory proteins such as UHRF1 and DNMT3L. DNMT3B isoforms are widely expressed, although some do not have active catalytic domains an...

Journal: :Development 2002
Kenichiro Hata Masaki Okano Hong Lei En Li

Genomic imprinting is regulated by differential methylation of the paternal and maternal genome. However, it remains unknown how parental imprinting is established during gametogenesis. In this study, we demonstrate that Dnmt3L, a protein sharing homology with DNA methyltransferases, Dnmt3a and Dnmt3b, but lacking enzymatic activity, is essential for the establishment of maternal methylation im...

Journal: :Cell reports 2016
Jiao Yang Renpeng Guo Hua Wang Xiaoying Ye Zhongcheng Zhou Jiameng Dan Haiying Wang Peng Gong Wei Deng Yu Yin ShiQing Mao Lingbo Wang Junjun Ding Jinsong Li David L Keefe Meelad M Dawlaty Jianlong Wang GuoLiang Xu Lin Liu

Ten-eleven translocation (Tet) family proteins convert 5-methylcytosine to 5-hydroxymethylcytosine. We show that mouse embryonic stem cells (ESCs) depleted of Tet1 and/or Tet2 by RNAi exhibit short telomeres and chromosomal instability, concomitant with reduced telomere recombination. Tet1 and Tet2 double-knockout ESCs also display short telomeres but to a lesser extent. Notably, Tet1/2/3 tripl...

Asaadi Tehrani G Ebrahimi B Koliforosh M, Shahhosseini M

Background: Ovarian tissue cryopreservation is a feasible method to preserve female reproductive potential, especially in young patients with cancer or in women at risk of premature ovarian failure. Vitrification has recently emerged as a new trend for biological specimen preservation. On the other hand, gene expression that changes during vitrification can influence oocyte maturation and need ...

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