Avian adenovirus was isolated from naturally infected birds during 1990 to 2003 in the vicinity ofKarachi, Pakistan. The virus is known to cause hydro-pericardium syndrome (HPS) in poultry. The mortalitywas recorded 20-50% in commercial broilers. Fifty field samples were collected; however, six samples wereselected for molecular studies. These isolates were grown and characterized by cell cultu...

The ocr protein, the product of gene 0.3 of bacteriophage T7, is a structural mimic of the phosphate backbone of B-form DNA. In total it mimics 22 phosphate groups over approximately 24 bp of DNA. This mimicry allows it to block DNA binding by type I DNA restriction enzymes and to inhibit these enzymes. We have determined that multiple ocr dimers can bind stoichiometrically to the archetypal ty...

The ocr protein, the product of gene 0.3 of bacteriophage T7, is a structural mimic of the phosphate backbone of B-form DNA. In total it mimics 22 phosphate groups over ~24 bp of DNA. This mimicry allows it to block DNA binding by type I DNA restriction enzymes and to inhibit these enzymes. We have determined that multiple ocr dimers can bind stoichiometrically to the archetypal type I enzyme, ...

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The photoactivatable modified oligonucleotides were used to investigate direct contacts formed by the type IIE EcoRII restriction endonuclease and T/A bases of its recognition site (5'-CCT/AGG). dimer consists a central catalytic core, made two C-terminal endonuclease-like domains (EcoRII-C) from different subunits, N-terminal effector DNA binding (EcoRII-N). According co-crystal structure isol...

 In this study the forward and reverse primers were designated to amplify the segments (~250 bps and ~650 bps) of the gene coding domains 1 and 4 of aerolysin of Aeromonas hydrophila. These two domains are involved in pathogenesis of the aerolysin gene. Sequences for two restriction enzymes, Pst I and Hind III, were included in the forward and reverse primers respectively. These restriction enz...