Conventional methods for preparation of megabase plant DNA require a protoplast preparation step (1—5). Preparation of protoplasts is tedious and time-consuming. In contrast isolation of nuclei from leaf tissue is simple. Many researchers have attempted to prepare megabase DNA from nuclei (e.g., 3-6) , but no success has been reported. As an alternative approach Guidet and Langridge (6, 7) desc...

The pre-mutagenic oxidative DNA base damage of 8-hydroxy-guanine is present in DNA isolated from cells and the amount present increases with exposure of cells to oxidative stress. The oxidative DNA base damage may be present before isolation of DNA or it may be produced during isolation and processing of DNA. We have found that the amount of oxidative base damage measured in DNA can be reduced ...

One of the greatest challenges when attempting to identify victims of mass fatality incidents is the analysis of DNA from bone. The bone is more difficult to process and extract for DNA. Additionally, the initial cleaning and sampling of the bone sample is a labor-intensive and time-consuming step prior to isolating DNA. Thus, it is difficult to adapt the current method for automation. In addit...

We have investigated the relationship between sexual isolation and DNA sequence divergence in the transformation (at locus rpoB) of a naturally competent strain of Bacillus subtilis. Using both genomic DNA and a PCR-amplified segment of gene rpoB as donor, we found that the extent of sexual isolation at locus rpoB was closely predicted, over three orders of magnitude, as a log-linear function o...

Nucleic acids belong to the most important molecules and therefore the understanding of their properties, function and behavior is crucial. Even though a range of analytical and biochemical methods have been developed for this purpose, one common step is essential for all of them - isolation of the nucleic acid from the from complex sample matrix. The use of magnetic particles for the separatio...

A procedure is described for the isolation of native DNA from protoplasts of ammi (Ammi visnaga) and carrot (Daucus carota) cells. Protoplasts were produced from 40 grams of fresh cells by enzyme hydrolysis and lysed with sodium dodecyl sulfate. The DNA was purified by treatment with pronase and ribonuclease. Final isolation was achieved by sucrose density gradient centrifugation.The melting te...

this study investigated the genetic diversity of morchella species using randomly amplified polymorphic dna markers. morchella species are an important group of edible mushrooms belonging to the family helveliaceae with medicinal and economical significance. in this study we have developed an efficient method of genomic dna isolation which was amplified by eight rapd primers to test the polymor...

Social isolation and loneliness increase the risk of death as much as well-established risk factors for mortality such as cigarette smoking and alcohol consumption. The underlying molecular mechanisms are poorly understood. In the present study, 3 months old male C57BL/6 mice were socially isolated by individual housing for another 3 months. At the age of 6 months, epigenetic changes were analy...

A polycephalic larva of Taeniidae family isolated from abdominal cavity of a great gerbil (Rhombomys opimus) from Golestan province, northern Iran, was subjected to molecular analysis. Genomic DNA from the larva was obtained using a DNA extraction tissue kit. Polymerase chain reaction was performed for amplification of the partial 12S rRNA, cytochrome c oxidase subunit 1 (cox1...

Several basic steps are performed during DNA testing regardless of the type of test being done. The general procedure includes: 1) the isolation of the DNA from an evidence sample containing DNA of unknown origin, and generally at a later time, the isolation of DNA from a sample (e.g., blood) from a known individual; 2) the processing of the DNA so that test results may be obtained; 3) the dete...