Clonorchis sinensis is a biological carcinogen inducing human cholangiocarcinoma, and clonorchiasis is one of the important endemic infectious diseases in East Asia. The present study investigated survival longevity of C. sinensis adult worms in various in vitro conditions to find the best way of keeping the worms longer. The worms were maintained in 0.85% NaCl, 1×PBS, 1×Locke's solution, RPMI-...

BACKGROUND Cultures of human ovarian granulosa lutein (hGL) cells are broadly used in experimental studies. The choice of the culture medium is important for the optimization of the conditions for culture of hGL cells. AIM To compare the efficiency of a basic salt solution and eight different defined media on the culture of hGL cells. MATERIALS AND METHODS Cultures of the HGL-5 cell line we...

The study aims to identify the phenotypic marker expressions of different human adult stem cells derived from, namely, bone marrow, subcutaneous fat, and omentum fat, cultured in different media, namely, DMEM-Low Glucose, Alpha-MEM, DMEM-F12 and DMEM-KO and under long term culture conditions (>P20). We characterized immunophenotype by using various hematopoietic, mesenchymal, endothelial marker...

Aggregation of the nanoparticles is a natural phenomena due to various biological and physical parameters but these aggregates are highly unstable. If would have been stable then it might be useful for some biomedical applications. To study optical properties photothermal heat generation metal nanopartilces, we present forced synthesis small gold nanorods in mixture Dulbecco's Modified Eagle's ...

Poly-Ether-Ketone (PEEK) is a bioactive material capable of replacing traditional metal and ceramic components in biomedical applications but still lacks some strength biological propertices compared with natural bone. In this research, calcium hydroxyapatite (cHAp) reduced Graphene oxide (rGO) were combined PEEK, different lattice porose structures produced to improve interfaces biocompatibili...

THE ENVIRONMENT AND THE LUNG 39$ achieved by prior culture for five days in DMEM + 0.5% FCS) and fibroblast cell counts determined after one, two, and four days. DMEM + 0.5% FCS ± asbestos incubated for four hours as controls. Supernatants from unexposed PAM had no influence on fibroblast replication rate. In contrast, supernatants from PAM exposed to asbestos significantly increased the rate o...

Neural progenitor cells (NPCs) of feline origin (cNPCs) have demonstrated utility in transplantation experiments, yet are difficult to grow in culture beyond the 1 month time frame. Here we use an enriched, serum-free base medium (Ultraculture) and report the successful long-term propagation of these cells. Primary cultures were derived from fetal brain tissue and passaged in DMEM/F12-based or ...

The aim of this experiment is to identify related genes for human umbilical mesenchymal stem cells transformation into nervous cells. After the human umbilical mesenchymal stem cells were treated with neuronal conditioned medium (NCM) for 9 days, the gene expression groups are compared to those only treated with DMEM. The related genes for cell cycles, the human umbilical mesenchymal stem cells...

The NMR spectra and data reported in this article refer to the research article titled "A simple and accurate protocol for absolute polar metabolite quantification in cell cultures using q-NMR" [1]. We provide the (1)H q-NMR spectra of cell culture media (DMEM) after removal of serum proteins, which show the different efficiency of various precipitating solvents, the solvent/DMEM ratios, and pH...