Somatic embryogenesis (SE) is a powerful tool for plant genetic improvement when used in combination with traditional agricultural techniques, and it is also an important technique to understand the different processes that occur during the development of plant embryogenesis. SE onset depends on a complex network of interactions among plant growth regulators, mainly auxins and cytokinins, durin...

To clone and examine expression profiles of DlRan genes during somatic embryogenesis in Dimocarpus longan Lour. Thirty cDNA sequences and two genomic sequences encoding DlRan proteins were isolated from longan embryogenic cultures. Structural analysis of DlRan genes revealed that the longan Ran gene family is more expanded than that of Arabidopsis. Expression analysis of DlRan genes during soma...

(In vitro somatic embryogenesis and adventitious root initiation have a common origin in eggplant (Solanum melongena L.)). Somatic embryogenesis was induced from cotyledon explants of eggplant cultured on MS medium supplemented with 54 μM NAA. Anatomical analysis of somatic embryo initiation and development was performed during the first four weeks. Proembryo formation was observed after the se...

The effect of 2, 4-D and TDZ concentrations on primary embryogenesis seem to be genotype dependent. There were significant differences between the control and the other treatments on somatic embryo production by genotype AMAZ 3-2. Genotype COCA 3370-5 did not show any significant difference among the treatments with 1mg/l 2, 4-D + 1μg/l TDZ, 1mg/l 2,4-D + 10μg/l TDZ and the control treatment. T...

Embryogenesis in cotton is a difficult task due its genome dependency. We used 3 cotton cultivars (Khandwa-2, G. Cot. 10, and BC-68–2) and Coker-312 as control for regeneration. Efficient somatic embryogenesis was induced in agronomically important Indian cotton cultivars, Khandwa-2 and G. Cot. 10. For callusing in all the cultivars, different media combinations were tried. Embryogenesis was in...