In this study, the ability of two antibiotics daptomycin and pantoyl lactone to inhibit growth of horse originated pathogen Streptococcus equi by disrupting plasma membrane of the bacterium was investigated using agar diffusion and micro tube dilution methods. The results obtained from this study indicated that daptomycin was more effective in inhibiting the growth of the pathogen in vitro than...

This paper evaluated the antibacterial effect of medlar and hawthorn compound extract in vitro. Water extract method and ethanol extraction method was adopted to prepare the compound extracts, and disc diffusion method and improved test tube doubling dilution method were used to conduct the antibacterial test on the two common pathogenic bacteria, Staphylococcus aureus and Klebsiella pneumonia,...

The end points of tube dilution tests for minimal inhibitory concentrations of miconazole and flucytosine against Candida albicans were difficult to evaluate because partial inhibition was noted over a wide range of antifungal concentrations. This problem was not encountered with amphotericin B. Partial inhibition of Candida arose because of reductions in yeast growth rate and of cell yield. Di...

A new antitumor antibiotic is produced in fermentation liquors of Streptomyces zelensis sp.n. The antibiotic is biologically active at extremely low concentrations. At 40 pg/ml, it inhibited 90% of the growth of L1210 cells in culture in tube dilution assays. The minimal inhibitory concentrations against Gram-positive bacteria is between 1 approximately 10 ng/ml, while these values for Gram-neg...

Twelve reagents were evaluated to develop a direct DNA extraction method suitable for PCR detection of foodborne bacterial pathogens. Many reagents exhibited strong PCR inhibition, requiring significant dilution of the extract with a corresponding reduction in sensitivity. Most reagents also exhibited much lower recovery of DNA from the gram-positive test organism (Listeria monocytogenes) than ...

conclusions we hope that turkey will be one of the brucellosis-free countries in near future with highlights from the current and further studies. materials and methods seroprevalence of brucellosis was examined in sera of 2913 patients who referred to igdır state hospital between february and december of the year 2010 by standard tube agglutination test method. results results were statistical...

Twenty years of Italian outdoor investigations on closed photobioreactors are discussed in this review. Many photobioreactor designs have been projected; some have been built, tested and patented. The Italian research approach from tubular (single tube and traditional loop) to flat and column and again to tubular photobioreactors (coil and loops) has improved microalgal yield. It increased from...

The physicochemical properties, composition and antimicrobial activity of cinnamon essential oil (Cinnamomum zeylanicum) were studied. The bioactivity of this essential oil against Paenibacillus larvae was analyzed by means of a combination of in vitro techniques, such as the tube dilution method and bioautography, a method employed to localize antibacterial activity on a chromatogram. Cinnamal...

A series of N-substituted imidazole derivatives was synthesized. Imidazole nucleus was reacted with ethylchloroacetate to form imidazole ester. Reaction of the imidazole ester (I) with different amines yields the desired products (1a- 1e). The compounds were characterized by FT-IR, (1)H-NMR and mass spectra. The synthesized compounds were evaluated for the antimicrobial activity against Staphyl...

The in vitro activity of lysostaphin against clinical isolates of Staphylococcus aureus was determined by conventional tube-dilution methods. For comparison, minimal inhibitory concentration (MIC) values were also determined for penicillin G, ampicillin, methicillin, ristocetin, vancomycin, and erythromycin. Phage type and penicillinase and coagulase production were determined for each isolate....