Hydrolysis is the most critical stage in high solids Temperature Phased Anaerobic Digestion (TPAD). In this paper two different Organic Fraction of Municipal Solid Waste (OFMSW) types were tested co-digestion with Digested Sludge (DS) at temperatures: 37, 55 and 65 °C. Volatile fatty acids (VFAs), soluble chemical oxygen demand (CODs) Biochemical Methane Production (BMP) measured calculated aft...

Bioaccessibility studies should be taken into account when evaluating the physiological effects of ingested compounds at the intestine level. Several in vitro digestion protocols have been described, with a wide range of experimental conditions but no optimised protocol exists. In order to fill in this gap, we evaluated the influence of three continuous factors (pH, incubation time, and enzyme ...

With an ultra-high surface area and abundant functional groups, graphene oxide (GO) provides an ideal substrate for the immobilization of trypsin. We demonstrated that trypsin could be immobilized on GO sheets assisted by polymers as molecular spacers to maintain the activity of the enzyme. And with the trypsin-linked GO as the enzyme immobilization probe, a novel microwave-assisted on-plate di...

Stability in simulated gastric fluid is regarded as an important parameter for the estimation of food allergenicity. In this study, the digestive stability of allergenic protein tropomyosin (TM) and other food proteins from mud crab in simulated gastric fluid (SGF) and simulated intestinal fluid (SIF) digestion assay system was investigated and compared by SDS-PAGE and Western blot. In SGF syst...

2.0 What are the challenges? There are two groups of ‘biogas generating’ organisms available within an Anaerobic Digestion process: acetoclastic methanogens and hydrogenotrophic methanogens. Food waste has a high nitrogen content due to the high protein component. During anaerobic digestion this generates a high level of ammonia which is toxic to the acetoclastic methanogens. Whilst biogas prod...

Various aspects of anaerobic digestion (AD) technology have been the focus of research in recent years. Shortening the digestion time with enhanced process efficiency is one of the integral concerns in AD technology. This study was conducted to investigate the feasibility of a two-phase anaerobic treatment system for unscreened dairy manure. Hydraulic retention time (HRT) and organic loading ra...

The potential of mesophilic anaerobic digestion for the treatment of fats of different origin through co-digestion with the organic fraction of municipal solid wastes (OFMSW) has been evaluated. Co-digestion process was conducted in a pilot plant working in semi-continuous regime in the mesophilic range (37 ◦C) and the hydraulic retention time (HRT) was 17 days. During the start-up period the d...

In the temperature range of 10 to 80 oC and in a batch digester, a typical activated sludge was digested aerobically. Reaction rate constants were determined by measuring the amounts of volatiles removed from the suspended solids at different time intervals during the process. The maximum value of the reaction rate constant (0.45 d ) occurred in the temperature range of 55-60 ̊C. Removal of indi...

Anaerobic digestion of sewage sludge (SS) and co-digestion with cheese whey (CW) was evaluated in batch conditions under mesophilic and thermophilic regimens. Results indicated that the co-digestion can be successfully attained despite some inhibitory stages that may be initially present due to the accumulation of volatile fatty acids. Degradation of the mixture SS-CW followed a similar pattern...

High pressure and temperature were combined with the convenient mixture of acids to perform an effective digestion of samples from aquatic animals for phosphorus (P) analysis. Our results indicate that digestion in high-pressure bombs combined with concentrated acidic media can be a convenient way to yield all P stored in samples, and to produce accurate recoveries. Phosphorus recovery of a cer...