A simple method for the isolation of pure and high-yield DNA from whole blood, suitable for restriction enzyme digestion, is described. The steps of the procedure are as follows: cell lysis with NH4Cl, NaHCO3, EDTA; digestion with proteinase K in the presence of SDS; extraction with phenol-chloroform-isoamyl alcohol; and precipitation with ethanol. The 260 nm/280 nm absorbance ratio showed a me...

The distribution of fibrinolytic activity in tissues may be studied by the technique of fibrinolysis autography (Todd, 1958, 1959, 1960). This is a histological method in which a section of fresh tissue is incubated in contact with a thin layer of bovine fibrin. During incubation discrete zones of digestion appear in the fibrin, related presumably to concentrations of fibrinolytic enzymes in th...

The production and application of compost potentially contaminate the environment with heavy metals. The heavy metal contents of composts must be exactly determined. Therefore, this study aims to understand the heavy metal contents in composts, using different digestion methods and to recommend the most appropriate digestion method making this measurement. Nine composts from different sources, ...

the effect of temperature on the main feeding parameters of mnemiopsis leidyi from the southern caspian sea was studied in 2002. the clearance rates and daily rations were estimated from laboratory experiments in a wide range of temperatures from 12 to 27 ?c for m. leidyi of 12?17 mm in length. clearance rate values changed from 52.5 to 107.3 ml ind-1 h-1. the coefficient q10 in temperature 12 ...

the effect of organic loading rate (olr) provided by hydraulic retention time (hrt) and influent chemical oxygen demand (codin) on the performance of an up-flow anaerobic sludge fixed film (uasff) bioreactor treating palm oil mill effluent (pome) was studied. anaerobic digestion of pome was modeled and analyzed with two variables i.e. hrt and codin. experiments were conducted based on a general...

To the Editor: Hemochromatosis is a common autosomal recessive genetic disorder of iron metabolism. In the United Kingdom, more than 90% of patients with hereditary hemochromatosis are homozygous for the C282Y mutation of the HFE gene, but other single-nucleotide polymorphisms (SNPs) within the HFE gene, namely H63D and S65C, have also been associated with the hemochromatosis phenotype. Various...

Rapid and efficient construction of expression vectors and subsequent transformation are basic recombinant methods for the investigation of gene functionality. Although novel cloning methods have recently been developed, many laboratories worldwide continue to use traditional restriction digestion-ligation methods to construct expression vectors owing to financial constraints and the unavailabi...

This study presents on-tissue proteolytic digestion using a microwave irradiation and peptide extraction method for in situ analysis of proteins from spatially defined regions of a tissue section. The methodology utilizes hydrogel discs (1 mm diameter) embedded with trypsin solution. The enzyme-laced hydrogel discs are applied to a tissue section, directing enzymatic digestion to a spatially co...

Objective: To compare the characteristics of human gingival fibroblasts (hGFs) obtained by tissue-explant and enzyme-digestion methods. Methods: The hGFs were isolated using tissue explants and enzyme digestion methods and identified by immunocytochemical analysis. Cell-surface molecules were detected by flow cytometry analysis. The colony-forming activity was examined by CFU-F assay. Oil red O...