In this study, two new duplex PCR methods based on the groEL gene were developed and investigated for the diagnosis of rickettsiae. The first duplex PCR assay amplified the 229-bp and the 366-bp DNAs of 6 strains including typhus group (TG) and spotted fever group (SFG) rickettsiae, and 5 scrub typhus group (STG) rickettsiae, respectively. The second duplex PCR assay amplified the 397-bp and th...

In the era of the Human Genome Project, quantitation of gene expression by tumor/host cells is of paramount importance to investigate gene patterns responsible for cancer development, progression and response/resistance to treatment. Quantitative real-time PCR (qrt-PCR) technology has recently reached a level of sensitivity, accuracy and practical ease that support its use as a routine bioinstr...

Proline accumulation is a well-known response to water deficits in leaves. The primary cause of accumulation is proline synthesis. Delta(1)-Pyrroline-5-carboxylate reductase (PCR) catalyzes the final reaction of proline synthesis. To determine the subcellular location of PCR, protoplasts were made from leaves of Pisum sativum L., lysed, and fractionated by differential and Percoll density gradi...

Abstract Grey oyster mushroom is the most often farmed and marketed type of for household consumption in Malaysia. The cultivation takes a long time due to its complicated mycelium growth. Several treatments were introduced such as acoustic sound treatment increase growth rate quality crop, but there no in-depth study regarding genetic expression genes affected. This aims evaluate Differential ...

Abstract During curing of thermosetting resins the technologically relevant properties binders and coatings develop. However, is difficult to monitor due multitude chemical physical processes taking place. Precise prediction specific technological based on molecular very difficult. In this study, potential principal component analysis (PCA) regression (PCR) in Fourier transform infrared (FTIR) ...