Extraction of intact quality DNA from plant tissues, especially those rich in secondary metabolites, is often challenging. Literally, hundreds of different DNA isolation protocols from various plant species have been published over the last decades. Although many commercial DNA isolation kits are convenient and designed to be safe, their cost and availability cause limitations in small molecula...

Little is known about the immunogenicity of RABORAL V-RG(®) (V-RG), an oral rabies vaccine, in raccoon kits (Procyon lotor). The objectives of this study were to characterize the immunogenicity of V-RG in young kits and investigate the potential impact of maternal antibodies on response to vaccination of nursing raccoon kits. Raccoon kits (n=30) were vaccinated at either 3 weeks of age, 7 weeks...

Meningitis is a severe and potentially fatal form of tuberculosis. The diagnostic workup involves detection of acid-fast bacilli (AFB) in the cerebrospinal fluid (CSF) by microscopy or culture, however, the difficulty in detecting the organism poses a challenge to diagnosis. The use of the polymerase chain reaction (PCR) in the diagnostic approach to Mycobacterium tuberculosis (MTB) meningitis ...

Six kinds of assay kits based on different detecting principle for PSA were evaluated. Polyclonal antibody was used in 2 kits (Eiken, Markit-F) and monoclonal antibody in 4 kits (Ball Elsa, Delfia, Markit-M, Tandem-R). To evaluate their characteristics, sera of 12 female patients, 5 prostatectomized patients and 2 high stage prostate cancer patients were measured by these kits. On the female se...

In this study, we describe a case of severe toxicity by lincomycin contamination in feed on farm housing 1800 rabbit does. The used two-batch system, with parities 24 and 3 d ago, respectively. rabbits that had consumed the developed anorexia 24-48 h later, followed enteritis-diarrhoea death. Mortality was >70 % does, >50 28-day-old kits. None 7-day-old kits died from cause. A total 125 n...

The non-invasive, cost-effective malaria tests that minimize the need for blood collection are the need of time. The diagnosis of malaria in biological fluids other than blood would be a valuable approach in case management and epidemiological studies of malaria. Many rapid diagnostic kits are available in the market but till now there is no kit for diagnosing malaria from urine and saliva samp...

Objectives: The invasive nature of the current malaria diagnostic techniques impairs compliance to diagnosis, especially for on-field detection. Adapting non-invasive methods biological sample collection rapid diagnosis infections may provide a more efficient approach case management and epidemiological studies malaria. This study was designed evaluate detection Plasmodium falciparum Histidine-...

Four techniques for estimating serum T4 and three for estimating serum T3 have been investigated and found to be satisfactory in routine use. Normal ranges for each techniques have been established. Estimation of serum T3 by the commerical kits tested appears to have a high discriminant value in the diagnosis of hyperthyroidism, although the diagnostic definition used inevitably enhances the ap...