The profile and normal concentrations of nucleotide metabolites in human saliva and reproducibility of these determinations were analyzed. Samples of human saliva collected from healthy individuals at weekly intervals, were deproteinized and analysed for the content of adenine nucleotides and their metabolites by reversed-phase HPLC. Initial ATP, hypoxanthine and uric acid concentrations were 0...

When chromatin was isolated from cells infected with human cytomegalovirus, the virus DNA remained with the chromatin fraction. If deproteinized virus DNA was added to either isolated nuclei or chromatin, the DNA was lost during the chromatin isolation. When isolated chromatin from cytomegalovirus-infected cells was banded in isopycnic metrizamide gradients, a single peak with a density of 1.18...

The experiments described herein demonstrate the plaque-forming cell response of C3H/HeJ mice can be suppressed by a Boivin type lipopolysaccharide (LPS) and a deproteinized glycolipid. Suppression was observed both in vivo and in vitro, and could be transferred to normal cells in coculture experiments. This newly discovered effect of LPS in C3H/HeJ mice indicates that the adjuvant and inhibito...

A capillary electrophoretic method (CE) with ultraviolet absorption detection for baclofen in human plasma was developed. The separation was obtained by CE with 50 mM borate buffer at pH 9, followed by detection with an ultraviolet detector at 225 nm. The plasma samples were deproteinized with acetonitrile to remove proteins and to induce sample stacking. With stacking, the limit of detection (...

An accurate, reproducible colorimetric method for determining minute amounts of alkylating agents in biologic fluids has been developed. Utilizing this method, we have been able to detect less than 0.1 g. of nitrogen mustard (HN2), or Thio-TEPA in 1 ml. of plasma, lymph, urine, or tissue homogenate. The biologic fluid was deproteinized with zinc sulfate, barium hydroxide, and methanol; the supe...

Incubation o f deproteinized larval cuticle (chitin flakes) with purified arylphorin (calliphorin) or larval haemolymph o f Calliphora vicina resulted in the formation o f a chitin-protein complex. Enzymatic oxidation o f N-ß-alanyldopamine (N D A B ) in the presence o f chitin flakes or the chitin-protein complex, resulted in various degrees o f cross-linking o f N BA D quinone formed with chi...

Choline was isolated from deproteinized plasma by cation-exchange chromatography. Isolated choline was directly converted to the 3,5-dinitrobenzoate derivative and was analyzed by paired-ion high-performance liquid chromatography with UV detection at 254 nm. An internal standard, 3-hydroxy-N,N,N-trimethylpropanaminium iodide was used for quantitation of plasma choline. Linearity was achieved fr...