The Escherichia coli sn-1,2-diacylglycerol (DAG) kinase has been successfully expressed in COS cells. The E. coli dgkA locus which contains the coding sequences for DAG kinase was subcloned into an eukaryotic expression vector, pMT2. COS cells transfected with the vector pMT2dgk expressed the DAG kinase as shown by Western analysis. Immunofluorescence studies revealed that the E. coli DAG kinas...

Diacylglycerol kinases (DGKs) terminate diacylglycerol (DAG) signaling and promote phosphatidic acid (PA) production. Isoform specific regulation of DGKs activity and localization allows DGKs to shape the DAG and PA gradients. The capacity of DGKs to constrain the areas of DAG signaling is exemplified by their role in defining the contact interface between T cells and antigen presenting cells: ...

OBJECTIVE Decreased expression of diacylglycerol kinase delta (DGKδ) has been linked to insulin resistance in humans and mice and it is abundantly expressed in adipose tissue. Therefore, its role in adipogenesis was examined. DESIGN AND METHODS 3T3-L1 pre-adipocytes were generated in which DGKδ expression had been knocked down and the effect of this on adipogenesis was determined. Lipidomic a...

This work analyzed the process of lipid storage in fat body of larval Manduca sexta, focusing on the role of lipid transfer particle (LTP). Incubation of fat bodies with [(3)H]diacylglycerol-labeled lipophorin resulted in a significant accumulation of diacylglycerol (DAG) and triacylglycerol (TAG) in the tissue. Transfer of DAG to fat body and its storage as TAG was significantly inhibited (60%...

We apply MCMC to approximately calculate (i) the ratio of directed acyclic graph (DAG) models to DAGs for up to 20 nodes, and (ii) the fraction of chain graph (CG) models that are neither undirected graph (UG) models nor DAG models for up to 13 nodes. Our results suggest that, for the numbers of nodes considered, (i) the ratio of DAG models to DAGs is not very low, (ii) the ratio of DAG models ...

We describe a new paradigm for implement­ ing inference in belief networks, which con­ sists of two steps: (1) compiling a belief network into an arithmetic expression called a Query DAG (Q-DAG); and (2) answering queries using a simple evaluation algorithm. Each non-leaf node of a Q-DAG represents a numeric operation, a number, or a symbol for evidence. Each leaf node of a Q-DAG repre­ sents t...

We describe a new paradigm for implementing inference in belief networks, which consists of two steps: (1) compiling a belief network into an arithmetic expression called a Query DAG (Q-DAG); and (2) answering queries using a simple evaluation algorithm. Each node of a Q-DAG represents a numeric operation, a number, or a symbol for evidence. Each leaf node of a Q-DAG represents the answer to a ...

PURPOSE In epidemiologic and animal studies, a high fat diet (HFD) has been shown to be associated with lower bone mineral density (BMD) and a higher risk of osteoporotic fractures. Meanwhile, consuming a HFD containing diacylglycerol (DAG) instead of triacylglycerol (TAG) is known to offer metabolically beneficial effects of reductions in body weight and abdominal fat. The purpose of this stud...

Extracorporeal formation of mineralized bone-like tissue is still an unsolved challenge in tissue engineering. Embryonic stem cells may open up new therapeutic options for the future and should be an interesting model for the analysis of fetal organogenesis. Here we describe a technique for culturing embryonic stem cells (ESCs) in the absence of artificial scaffolds which generated mineralized ...

We introduce linear expressions for unrestricted dags (directed acyclic graphs) and finite deterministic and nondeterministic automata operating on them. Those dag automata are a conservative extension of the Tu,u-automata of Courcelle on unranked, unordered trees and forests. Several examples of dag languages acceptable and not acceptable by dag automata and some closure properties are given.