HUT-78 cells were infected with a reverse transcriptase (RT)-positive supernatant of a culture of peripheral blood lymphocytes (PBL) from an AIDS patient and then cloned. Of these clones, two have been isolated and characterized. Clone D10 is persistently and productively infected with an HIV variant. The clone F12, in spite of the presence of an integrated full-length HIV provirus, does not re...

Of 113 lactobacilli isolated from radurized (5 kGy) minced meat, 7 Lactobacillus sake strains, 1 L. curvatus strain, and 1 L. farciminis strain were used for radiation resistance studies in a semisynthetic substrate (i.e., modified MRS broth). Five reference Lactobacillus spp., one Staphylococcus aureus strain, and one Salmonella typhimurium strain were used for comparative purposes. All L. sak...

The aim of this study was to synthesize a series of quinoline-pyrimidine hybrids and to evaluate their in vitro antimalarial activity as well as cytotoxicity. The hybrids were brought about in a two-step nucleophilic substitution process involving quinoline and pyrimidine moieties. They were screened alongside chloroquine (CQ), pyrimethamine (PM) and fixed combinations thereof against the D10 a...

foot and mouth disease (fmd) is the most contagious disease of cloven-hoofed animals, causingsignificant economical losses in livestock animals. in this study fmd virus type a87/irn was cultured andmultiplied on bhk21 cells. the fmd virus was titrated by the tissue culture infection dose50 method(tcid50 /ml), the virus titration was 107.5.. the fmd virus samples were irradiated and inactivated ...

The rotational spectra of m-xylene, (CH3)2C6H4, and of m-xylene-d10, (CD3)2C6D4, have been recorded between 6 and 26.5 GHz using pulsed beam Fourier transform microwave spectroscopy. The clue for the assignment of the internal rotation multiplets was the inertial defect derived from the A1A1 species transitions. The rotational constants for m-xylene and m-xylene-d10 are A = 3572.1117(1) MHz / 2...

Pichia pastoris is commonly used to express and secrete target proteins, although not all recombinant proteins can be successfully produced. In this study, we used methyl parathion hydrolase (MPH) from Ochrobactrum sp. M231 as a model to study the importance of the N-terminus of the protein for its secretion. While MPH can be efficiently expressed intracellularly in P. pastoris, it is not secre...

Series of bisquinolines 4-15 and bispyrrolo[1,2a]quinoxalines 16-20 containing various polyamine linkers were synthesized. The aqueous solubility and distribution coefficient were experimentally determined. The compounds were screened for antimalarial activity alongside chloroquine against D10 and Dd2 strains of Plasmodium falciparum. The growth inhibitory effects of biscompounds 4-9 were asses...

Objective: The objective of this study was to compare and correlate the analytical performance of D10 Hemoglobin Testing System based on Cation Exchange HPLC and Roche Hitachi 902 Immunoturbidimetric method. Subjects and Methods: A total of 110 patients of Type 2 Diabetes Mellitus were included in the study. HbA1c was determined using D-10 Hemoglobin Testing system and Roche Hitachi 902 Analyze...

The distribution of end-plate lesions (Schmorl's nodes) and their relationship to bone density and disc degeneration have been studied in 50 post-mortem spines below D9 in subjects aged 13-96 years. Lesions were present in 76% of cases with a predominance in males. They were found more frequently in the lower than in the upper vertebral end-plate. They were also more common and more severe in t...