The immune response against two herpesviruses has been determined in sera and matched synovial fluids of patients with rheumatoid arthritis (RA) and compared with that of a healthy control population. The increased level of antibody to Epstein-Barr virus (EBV) induced antigens in patients with RA resembles the antibody pattern observed against cytomegalovirus (CMV) induced antigens, which sugge...

Cytomegalovirus antibodies were measured in human sera by a nuclear anticomplement immunofluorescence test that used as antigen the isolated nucleic of virus-infected fibroblasts cells lysed in distilled water. The method exhibited less nonspecific fluorescence than either a conventional whole-cell anticomplement immunofluorescence test or an indirect fluorescent antibody test applied to the sa...

The titres of IgG antibody against "late antigens", "immediate early antigens", and "early antigens" induced by cytomegalovirus (CMV) and IgM antibody against "late antigens" induced by CMV were analysed in 67 geriatric subjects by immunocytochemical techniques. The titres obtained were compared with those of an adult control population. Significantly increased titres of IgG antibody against in...

A group of 120 sera from blood donors was screened by complement fixation and commercially available immunofluorescence, solid-phase fluorescence immunoassay, enzyme-linked immunosorbent assay, and indirect hemagglutination tests. Twenty-four of the sera were positive by three or more of the five tests and judged to be true positives; 89 were negative by three or more of the tests and considere...

OBSTETRICS & GYNECOLOGY Background Cytomegalovirus Cytomegalovirus is a ubiquitous double-stranded DNA herpesvirus that is transmitted by sexual contact or direct contact with infected blood, urine, or saliva. After an incubation period of 28–60 days (mean, 40 days), CMV infection induces immunoglobulin M (IgM) antibody production followed by an immunoglobulin G (IgG) antibody response. Viremia...

A latex agglutination test for determination of antibody against cytomegalovirus was compared with five other methods: a solid-phase fluorescent immunoassay, an indirect hemagglutination test, two solid-phase enzyme immunoassays, and an indirect fluorescent-antibody method, with sera collected from 210 random blood donors. Of the sera tested, 28% were positive for anti-cytomegalovirus by concor...

The recent article by Mazzulli et al. (4) further demonstrates the increasing interest of virologists and microbiologists in the cytomegalovirus antigenemia (CMV-Ag) assay. Mazzulli and coworkers report that the CMV-Ag assay is more sensitive than the shell vial-indirect immunofluorescence assay (SVAIFA), perhaps implying that the direct antigen detection assay should be considered the method o...

background and aims: primary infections of rubella and human cytomegalovirus (hcmv) can lead to severe complications in pregnancy. the screening of rubella and hcmv in pregnant women is not routinely carried out in iran. this cross sectional study aimed to investigate the seroprevalence of hcmv and rubella infections in neonates with and without congenital defects. materials and methods: this s...

cytomegalovirus (cmv) infection can cause colitis which is usually manifested with fever, weight loss, anorexia and abdominal pain.watery diarrhea can be the only manifestation. cmv colitis usually can not be diagnosed according to the clinical findings. moreover, endoscopic appearance can exactly mimic pseudo-membranous colitis. here by, we are presenting a patient with fever and watery diarrh...