نتایج جستجو برای: cytochemistry
تعداد نتایج: 1085 فیلتر نتایج به سال:
There was sharp opposition between ‘analomists and ‘physiologists’ when I was a medical student in the University of Urussels, some 60 years ago. This split was cxcmplificd by the prcsencc of two separate buildings. called respcctivcly Institutes of Anatomy and of Physiology, in the newly crcctcd Medical School. The first housed embryologists, histologists and pathologists, the second physiolog...
A cytochemical study of the lysosomal enzyme beta-glucuronidase in 60 cases of acute leukaemia has shown a qualitative difference in the cytoplasmic distribution of the enzyme between blast cells of the lymphoid and myeloid cell series. This difference provides a useful additional method for cytochemical classification of cell type and is superior in this respect to the other lysosomal enzymes ...
An Mg(2+)-dependent and a K(+)-stimulated adenosine triphosphatase were localized by cytochemistry at or near both surfaces of the cytoplasmic membrane of Myxococcus xanthus. An alkaline and an acid phosphatase resided at the external surface of the membrane or in the periplasm. All enzymes could be extracted from partially fixed cells with Mg(2+)-deficient buffers. Suboptimal external phosphat...
1. In the epidermis non-specific esterase activity outlines a strongly reactive band between the stratum granulosum and the stratum corneum. In the epidermis of the palm, there is no such esterase-rich band. 2. The outer sheath of active hair follicles has strong enzyme activity. The degenerating hair bulb in catagen follicles is very strongly reactive, and clusters of cells around the hair clu...
In contrast to the modern science of molecular biology, cytology and cytochemistry of human blood cells have a long history. Some of the principal mechanisms of protein biosynthesis were already discovered with cytological methods in the 19th century. In 1899, a young French anatomist, GANIER (8), demonstrated in his thesis the relationship between specific morphologicd changes in the cytoplasm...
This paper reports the synthesis of 14 diazophthalocyanins containing Mg, Cu, or Pb as the chelated metal. To assess the usefulness of these compounds for fine structural cytochemistry, the relative coupling rates with naphthols were tested as well as the solubility of the resulting azo dyes. Three of the diazotates were reacted with tissue proteins in aldehyde-fixed material, and the density i...
A new technique for the quantitative estimation of glycolytic and respiratory enzyme activity in intact and unfixed bone marrow and peripheral blood cells is described. The method gives a two- to three-fold increase in demonstrable enzyme activity per cell compared with existing techniques using fixed marrow film preparations, and is particularly applicable to the study of relatively fragile ce...
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