The emerging discipline of pharmacogenomics applies genomic technologies to predict individuals' responses to therapeutic drugs based on the genetic sequences of drug targets and enzymes involved in drug metabolism. The first diagnostic test for genotyping two important drug metabolizing enzymes (CYP450 2D6 and 2C19) has been FDA approved. This assay involves PCR amplification and identificatio...

Resolution of inflammation has emerged as an active process in immunobiology, with cells of the mononuclear phagocyte system being critical in mediating efferocytosis and wound debridement and bridging the gap between innate and adaptive immunity. Here we investigated the roles of cytochrome P450 (CYP)-derived epoxy-oxylipins in a well-characterized model of sterile resolving peritonitis in the...

Oyekan, A. O., K. McAward, J. Conetta, L. Rosenfeld, and John C. McGiff. Endothelin-1 and CYP450 arachidonate metabolites interact to promote tissue injury in DOCAsalt hypertension. Am. J. Physiol. 276 (Regulatory Integrative Comp. Physiol. 45): R766–R775, 1999.—Inhibition of cytochrome P-450 (CYP450) enzymes with cobalt chloride (CoCl2) prevented hypertension, organ hypertrophy, and renal inju...

Background/Aims: Cardiovascular events are common in patients with renocardiac syndrome. A direct link between endothelial dysfunction and increased cardiovascular events has not been established for this syndrome; however, a causal role could be attributed to modulations of Cytochrome P450-mediated (CYP450) eicosanoid metabolites including Epoxyeicosatrienoic (EETs), Dihydroxyeicosatrienoic (D...

O(6)-Benzylguanine (BG) effectively inactivates the DNA repair protein O(6)-alkylguanine-DNA alkyltransferase and enhances the effectiveness of alkylating agents, such as 1,3-bis(2-chloroethyl)-1-nitrosourea and temozolomide, in vitro and in vivo. BG is presently in clinical trials with 1,3-bis(2-chloroethyl)-1-nitrosourea and temozolomide. Preclinical data demonstrate that BG enhances the sens...

OBJECTIVE To determine cytochrome P450 (CYP450) and cyclooxygenase (COX) expression and metabolite regulation and renal damage in the early stages of obesity-related hypertension and diabetes. RESEARCH METHODS AND PROCEDURES Obese and lean Zucker rats at 10 to 12 weeks of age were studied. Blood pressure was measured in the conscious state using radiotelemetry. Blood glucose levels and body w...

INTRODUCTION Diabetes affects more than 9% of the adult population worldwide. Patients with type 2 diabetes mellitus (T2DM) show variable responses to some drugs which may be due, in part, to variability in the functional activity of drug-metabolising enzymes including cytochromes P450 (CYP450s). CYP450 is a superfamily of enzymes responsible for xenobiotic metabolism. Knowledge must be gained ...

O-Benzylguanine (BG) effectively inactivates the DNA repair protein O-alkylguanine-DNA alkyltransferase and enhances the effectiveness of alkylating agents, such as 1,3bis(2-chloroethyl)-1-nitrosourea and temozolomide, in vitro and in vivo. BG is presently in clinical trials with 1,3-bis(2chloroethyl)-1-nitrosourea and temozolomide. Preclinical data demonstrate that BG enhances the sensitivity ...