(Suggestions are based on Level III and IV evidence) • In children treated with cyclosporin, 2 h peak cyclosporin level (C2) monitoring should be used in the initial post-transplant period, as the C2 level is a better predictor of cyclosporin exposure than C0 monitoring (Level III evidence). Limited data suggest that the C2 level should be maintained above 1500 ng/mL in the early post-transplan...

Cyclosporin synthetase, a multifunctional polypeptide, catalyses the biosynthesis of the set of natural cyclosporins. We report that this enzyme is also capable of introducing a beta-alanine into position 7 or 8 of the ring instead of the alpha-alanines present at these positions in cyclosporin A. This leads to 34-membered rings in contrast to the 33-membered ring of the cyclo-undecapeptide cyc...

Anti-islet cell and anti-insulin antibody production was studies over a 12-mo period in 82 recently diagnosed diabetics randomly receiving either cyclosporin or placebo. Cyclosporin had only minimal effects on the production of anti-islet cell antibodies whether directed to islet cytoplasmic (immunofluorescence) or membrane (cytotoxicity assay) antigens even in patients undergoing remission. Th...

Changes of the biliary canalicular membrane lipid content can affect membrane fluidity and biliary lipid secretion in rats. The immunosuppressant cyclosporin A is known to cause intrahepatic cholestasis. This study investigated whether cyclosporin A influenced canalicular membrane fluidity by altering membrane phospholipids or transporter expression. In male Sprague-Dawley rats, a bile-duct can...

range of other conditions, including Another important aspect in its distribution is that it is highly bound to psoriasis, rheumatoid arthritis, and erythrocytes and plasma lipoproteins. At low blood concentrations (below asthma 80 nmolIl (100 ng/ml)) and when the packed cell volume is low (for example, in patients with kidney failure) the contribution of the plasma bound fraction becomes more ...

This study has shown low dose cyclosporin A to be effective in the treatment of severe psoriasis. Conventional treatment had failed to control the disease in the 10 patients studied, and this suggests that cyclosporin is more effective than other drugs that are currently available. Indeed, nine of the 10 patients said that cyclosporin was the most efficacious and tolerable treatment that they h...

Because cyclosporin A rapidly changes its distribution in blood with changes in temperature, sample preparation affects results for it as measured in plasma. If whole blood is stored at either 4 degrees C or room temperature, results for cyclosporin A in the plasma are lower than in whole blood stored at 37 degrees C and centrifuged at this temperature. Re-equilibration of the former to 37 degr...

Prednisone, prednisolone, and methylprednisolone are currently administered in association with cyclosporin A in the postoperative treatment of transplant patients. The aim of this work was to evaluate the effects of these corticosteroids on the expression of several forms of cytochromes P450 (P450), including P450 1A2, 2D6, 2E1, and 3A, and on cyclosporin A oxidase activity in human liver. For...

AIMS To describe management and clinical outcomes of serpiginous choroidopathy treated primarily with cyclosporin at a tertiary uveitis referral centre METHODS A case series of 14 eyes of seven patients with serpiginous choroidopathy with follow up ranging from 1.3 to 13 years is described. All patients had fundus lesions consistent with serpiginous choroidopathy, were investigated for system...

BACKGROUND Switching to cyclosporine A may result in a reversion of tacrolimus-induced diabetes mellitus. However, mechanisms underlying such a reversion are still unknown. METHODS Obese Zucker rats were used as a model for tacrolimus-induced diabetes mellitus. A cohort of 44 obese Zucker rats received tacrolimus for 11 days (0.3mg/kg/day) until diabetes development; then: (a)22 rats were eut...