Background: Mammalian Cell lines are the main expression system for the production of recombinant therapeutic proteins. Optimization of cell culture condition is performed via alteration in different parameter. Cell culture media plays an important role in cell cycle because of compounds such as amino acids, vitamins, inorganic salts, glucose, and serum as a source of growth factors, trace elem...

cell banking is long –term storing of cell stocks that requires intensive care and maintenance to ensure its adequate quality. the main goal of a biological bank is preparing a repository source of cell culture system in a global manner. on the other hand, cell culture is an invaluable tool for investigators in numerous fields. successful maintenance of cells requires knowledge and practice of ...

three differentes continous cell lines (am 57. hela • vero) and primary cell culture of human embryo kidney are compared with regard to their .susceptibility for isolation of mumps v i r u s from saliva and cerebrospinal fluid. for this purfose the vero cell line appeared to be more suitable among the studied cell systems regarding the following reasons: it allows the highest pourcentage of mum...

The aim of this study was to compare invasive and non-invasive strains of Shigella flexneri isolated from Tehran by a 120 kDa protein band by SDS-PAGE, electron microscopy of cell culture and Congo red dye methods. Methods: S. flexneri strains were isolated by standard bacterial methods from fecal specimens of children attending to the 3 children’s hospitals. Phenotype analysis for screening v...

background: this study was designed to develop a new co-culture system, assess the effect of luteinizing hormone (lh) using sequential media to promote development and increase the quality of 2-cell murine embryos through the 8-16 cell stage to morula and blastocyst stages. materials and methods: monolayers for co-culture were prepared from ovine oviduct epithelial cells (ooec) in dmem/f12 medi...