In this paper, the functional state modelling approach is validated for modelling of the cultivation of two different microorganisms: yeast (Saccharomyces cerevisiae) and bacteria (Escherichia coli). Based on the available experimental data for these fed-batch cultivation processes, three different functional states are distinguished, namely primary product synthesis state, mixed oxidative stat...

Microalgal lipid is a promising feedstock for biodiesel production. Effect of cultivation temperature on the growth and lipid accumulation properties of a freshwater microalga Scenedesmus sp. LX1 was studied. Scenedesmus sp. LX1 could grow in a wide range of temperature (10∼30°C), and the growth activation energy was 49.3 kJ·mol(-1). The optimal temperature to produce microalgal biomass and lip...

Students’ scientific and technological ability for innovation is high-quality talents’ core and soul. Aiming at the actuality of students’ scientific and technological ability for innovation and the cognition and analysis about the cultivation of students’ scientific and technological ability for innovation, the thoughts and measures about the cultivation of students’ scientific and technologic...

Background Cultivation temperature is a key environmental parameter that influences cell growth and recombinant protein production. Recombinant CHO (rCHO) cells are usually cultivated at 37 °C. Although lowering culture temperature below 37 °C decrease specific growth rate, in many cases, the specific production rate, q, of CHO cells was not enhanced by lowering the culture temperature. Unlike ...

Cultivation of an important sub alpine-alpine medicinal and aromatic herb, Angelica archangelica Linn. (Apiaceae), was carried out at two different climatic zone at 2200 m asl (Pothivasa, PV) and 3600 m asl (Tungnath, TN) altitudes in Garhwal, North west Himalaya, India. These altitudes represent temperate and alpine region of the Himalaya. Since the species has a very few wild populations, cul...

A marine photosynthetic bacterium (PS88) identified as Rhodovulum sp. with flocculating ability was isolated from the sea sediment mud of a shrimp cultivation farm in Thailand. This bacterium flocculated in glutamate/malate medium during aerobic/dark or anaerobic/light cultivation. The flocculating ability was enhanced with the increase of NaCl concentration to 6% (w/v). Flocculation and growth...

Enterococcus faecalis is frequently recovered from refractory endodontic infections and has been implicated in endodontic treatment failures. This study compared real-time quantitative PCR (qPCR) assay to cultivation for E. faecalis detection and quantitation during endodontic treatment. A reverse-transcription PCR (RT-PCR) assay was also developed to detect the bacterium clinically in the viab...

A new approach using microtiter plate cultivation with on-line measurement of dissolved oxygen (DO) was applied for medium optimization of mammalian cell culture. Applying dynamic liquid phase balance, oxygen uptake rates were calculated from the DO level and used as an indicator for culture viability. The developed method was successfully applied to optimization of the concentration of glucose...

In the future, due to limited resources, a crisis of energy storing molecules (fuels), which are currently produced from crude mineral oil, is expected. One strategy to compensate a part of the oil defi ciency is the production of biodiesel from microalgal lipids. As model microorganism for lipid production microalgae Euglena gracilis was selected and photo-mixotrophic cultivation was performed...

The use of commercially available plastic syringes is introduced as disposable small-scale fixed bed bioreactors for the cultivation of implantable therapeutic cell systems on the basis of an alginate-encapsulated human mesenchymal stem cell line. The system introduced is fitted with a noninvasive oxygen sensor for the continuous monitoring of the cultivation process. Fixed bed bioreactors offe...